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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Azizian, Reza Nasser, Ahmad Askari, Hasan Taheri Kalani, Morovat Sadeghifard, Nourkhoda Pakzad, Iraj Amini, Razieh Mozaffari Nejad, Amir Sasan Azizi Jalilian, Farid |
| Description | Country affiliation: Iran Author Affiliation: Azizian R ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Nasser A ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Askari H ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Taheri Kalani M ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Sadeghifard N ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Pakzad I ( Department of Medical Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran); Amini R ( Research Center for Molecular Medicine, Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.); Mozaffari Nejad AS ( Research Center for Molecular Medicine, Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.); Azizi Jalilian F ( Research Center for Molecular Medicine, Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: azizijalilian@yahoo.com.) |
| Abstract | Pseudomonas aeruginosa is a ubiquitous organism which has emerged as a major public health threat in hospital environments. Overuse of antibiotics has significantly exacerbated the emergence of multi-drug resistant bacteria such as P. aeruginosa. Phages are currently being utilized successfully for aquaculture, agriculture and veterinary applications. The aim of this study was to isolate and characterize of lytic P. aeruginosa phage from sewage of Ilam, Iran. Phage was isolated from sewage that was added to the enrichment along with the host and subsequently filtered. Plaque assay was done by using an overlay method (also called the double agar layer method). Purified plaques were then amplified for characterization. Finally, RAPD-PCR method was conducted for genotyping and Transition electron micrograph (TEM) recruited to determine the morphology and phage family. The phage had high concentration and tremendous effects against a variety of clinical and general laboratory strains (ATCC15693) of P. aeruginosa. Among a set of primers in RAPD panel, only P2 and RAPD5 primers, were useful in differentiating the phages. TEM images revealed that the isolated phages were members of the Siphoviridae family. The phage effectiveness and specificity towards target bacteria and potential to control biofilm formations will be investigate in our further studies. |
| File Format | HTM / HTML |
| ISSN | 10451056 |
| Issue Number | 4 |
| Journal | Biologicals |
| Volume Number | 43 |
| e-ISSN | 10958320 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-07-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Immunology Pseudomonas Phages Physiology Pseudomonas Aeruginosa Virology Sewage Microscopy, Electron, Transmission Ultrastructure Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Pharmacology Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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