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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Arora, R. Bhatia, S. Sehrawat, A. Pandey, A. K. Sharma, R. Mishra, B. P. Jain, A. Prakash, B. |
| Spatial Coverage | India |
| Description | Country affiliation: India Author Affiliation: Arora R ( National Bureau of Animal Genetic Resources, P.O. 129, Karnal, 132001, Haryana, India.) |
| Abstract | Information is presented on the genetic polymorphism of the Type 1 intermediate filament wool keratin gene in 15 native Indian sheep breeds belonging to different agro-ecological regions of India. The study analyzed random blood samples of the 638 sheep by the PCR-RFLP technique. Restriction digestion analysis of a 480 bp PCR fragment of the first exon region with MspI revealed two allelic variants (M = 0.748 and N = 0.252) and three genotypes (MM = 0.543, MN = 0.410, and NN = 0.047) across the 15 sheep breeds. The allelic frequency differences for both alleles across the Indian breeds, irrespective of their geographic distribution, color pattern, and utility traits, were observed to be statistically insignificant by a chi-square test (P > 0.05). According to the pattern of occurrence of allelic variants (M > N), the Indian breeds exhibited similarity to some of the reported European sheep breeds. The average heterozygosity was 0.420, and none of the breeds deviated from Hardy-Weinberg equilibrium. The predominance of the M over the N allele supported its ancestry in Indian sheep too. |
| File Format | HTM / HTML |
| ISSN | 00062928 |
| Issue Number | 9-10 |
| Volume Number | 46 |
| e-ISSN | 15734927 |
| Journal | Biochemical Genetics |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2008-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Molecular Biology Intermediate Filaments Genetics Keratins Polymorphism, Genetic Sheep Alleles Animals Ecology Exons Genetic Markers Genetic Variation Genetics, Population Genotype India Metabolism Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics |
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