NDLI: Development and characterization of RAPD and microsatellite markers for genetic variation analysis in the critically endangered yellow catfish Horabagrus nigricollaris (Teleostei: Horabagridae).

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Development and characterization of RAPD and microsatellite markers for genetic variation analysis in the critically endangered yellow catfish Horabagrus nigricollaris (Teleostei: Horabagridae).

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Abdul Muneer, P. M. Sivanandan, Remya Gopalakrishnan, A. Basheer, V. S. Musammilu, K. K. Ponniah, A. G.
Spatial Coverage	India
Description	Country affiliation: India Author Affiliation: Abdul Muneer PM ( National Bureau of Fish Genetic Resources, Cochin Unit, CMFRI Campus, Cochin, Kerala, India. pmamuneer@gmail.com)
Abstract	Random-amplified polymorphic DNA (RAPD) and microsatellite markers were developed and used for the analysis of genetic variability in the critically endangered yellow catfish Horabagrus nigricollaris, sampled from the Chalakkudy River, Kerala, India. Eight RAPD and five microsatellite markers were detected to genotype the species. In RAPD, the 73 fragments were 20.55% polymorphic, whereas 4 polymorphic loci (80%) were obtained in microsatellites. In microsatellites, the number of alleles across the 5 loci was 1-5, and the range of heterozygosity was 0.25-0.5. The mean observed number of alleles was 2.4, and the effective number was 1.775 per locus. The average heterozygosity across all investigated samples was 0.29, indicating a significant deficiency of heterozygotes in this species. RAPD and microsatellite methods report a low degree of gene diversity and lack of genetic heterogeneity in the population of H. nigricollaris, emphasizing the need for fishery management, conservation, and rehabilitation of this species.
File Format	HTM / HTML
ISSN	00062928
Issue Number	1-2
Volume Number	49
e-ISSN	15734927
Journal	Biochemical Genetics
Language	English
Publisher	Springer
Publisher Date	2011-02-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecular Biology Catfishes Genetics Genetic Markers Microsatellite Repeats Random Amplified Polymorphic Dna Technique Methods Alleles Animals Dna Endangered Species Gene Frequency Genetic Heterogeneity Genetic Loci Genotype Heterozygote India Phylogeny Polymorphism, Genetic Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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Development and Characterization of RAPD and Microsatellite Markers for Genetic Variation Analysis in the Critically Endangered Yellow Catfish Horabagrus nigricollaris (Teleostei: Horabagridae)

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