NDLI: Molecular characterization of the major histocompatibility complex class Ia gene in the black-spotted frog, Pelophylax nigromaculata.

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Molecular characterization of the major histocompatibility complex class Ia gene in the black-spotted frog, Pelophylax nigromaculata.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gong, Jie Sun, Qi-Peng Xue, Fei Fang, Sheng-Guo Wan, Qiu-Hong
Spatial Coverage	China
Description	Country affiliation: China Author Affiliation: Gong J ( Key Laboratory of Conservation Biology for Endangered Wildlife, Ministry of Education and State Conservation Center for Gene Resources of Endangered Wildlife, and College of Life Sciences, Zhejiang University, Zijingang Campus, 388 Yu Hang Tang Road, Hangzhou, 310058, China.)
Abstract	The genes of the major histocompatibility complex (MHC) are attractive candidates for investigating the link between adaptive variation and individual fitness. We improved rapid amplification of cDNA ends to obtain the whole coding sequence of the MHC class Ia gene of the black-spotted frog (Pelophylax nigromaculata), the most common amphibian in China. We also used genome walking to characterize the partial introns adjacent to exon 3 of the MHC Ia gene. Based on the sequences obtained, we designed locus-specific primers to investigate the molecular polymorphisms of this species in southeast China. The MHC class Ia gene showed a high level of genetic diversity, indicating that this species retains a relatively high potential for survival, despite a population decline among frog species in general and many other amphibians.
File Format	HTM / HTML
ISSN	00062928
Issue Number	11-12
Volume Number	51
e-ISSN	15734927
Journal	Biochemical Genetics
Language	English
Publisher	Springer
Publisher Date	2013-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecular Biology Genes, Mhc Class I Polymorphism, Genetic Ranidae Genetics Amino Acid Sequence Amphibians Animals China Genetic Variation Molecular Sequence Data Multigene Family Open Reading Frames Phylogeny Polymerase Chain Reaction Sequence Alignment Sequence Analysis, Dna Methods Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Biochemistry Molecular Biology Ecology, Evolution, Behavior and Systematics

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Molecular Characterization of the Major Histocompatibility Complex Class Ia Gene in the Black-Spotted Frog, Pelophylax nigromaculata

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Molecular characterization of the major histocompatibility complex class Ia gene in the black-spotted frog, Pelophylax nigromaculata.

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Molecular Characterization of the Major Histocompatibility Complex Class Ia Gene in the Black-Spotted Frog, Pelophylax nigromaculata

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Molecular characterization of the major histocompatibility complex class Ia gene in the black-spotted frog, Pelophylax nigromaculata.