NDLI: Generation of a reporter mouse line expressing Akt and EGFP upon Cre-mediated recombination.

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Generation of a reporter mouse line expressing Akt and EGFP upon Cre-mediated recombination.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Elghazi, Lynda Weiss, Aaron J. Gould, Aaron P. Hegedus, Balazs Gutmann, David H. Bernal-Mizrachi, Ernesto
Description	Country affiliation: United States Author Affiliation: Elghazi L ( Division of Endocrinology, Department of Internal Medicine, Metabolism and Lipid Research, Washington University School of Medicine, Saint Louis, Missouri 63110-1010, USA.)
Abstract	The serine-threonine kinase Akt regulates multiple biological processes. An important strategy to study Akt signaling in different tissues is targeted activation of this pathway in vivo. The current studies describe the generation of a mouse model that combines a double reporter system with activation of a constitutively active form of Akt1 (caAkt) in a Cre-dependent manner. Before Cre recombination, these mice express LacZ during development as well as in most adult tissues. After Cre-mediated excision of the LacZ reporter, functionality of the transgene was demonstrated by expression of the caAkt mutant along with the second reporter, EGFP in different pancreatic compartments and in the nervous system. This animal model provides a critical reagent for assessing the effects of Akt activation in specific tissues. The lineage-tracing properties provide a useful tool to study the role of Akt signaling in regulation of differentiation programs during development and plasticity of mature tissues.
File Format	HTM / HTML
ISSN	1526954X
e-ISSN	1526968X
DOI	10.1002/dvg.20388
Journal	genesis
Issue Number	5
Volume Number	46
Language	English
Publisher	Wiley-Liss
Publisher Date	2008-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Genetics Discipline Developmental Biology Discipline Embryology Gene Transfer Techniques Genes, Reporter Green Fluorescent Proteins Genetics Integrases Proto-oncogene Proteins C-akt Recombination, Genetic Animals Fluorescent Dyes Biosynthesis Mice Mice, Inbred C57bl Mice, Transgenic Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Medicine Endocrinology

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