NDLI: Cre-mediated recombination in mouse Clara cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, HuaiGuang Cho, Sung Nam Evans, Christopher M. Dickey, Burton F. Jeong, Jae-Wook DeMayo, Francesco J.
Description	Country affiliation: United States Author Affiliation: Li H ( Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.)
Abstract	Clara cells are nonciliated secretory cells lining the respiratory epithelium and are easily identified by the expression of Clara cell secretory protein (CCSP). To investigate molecular mechanism(s) regulating Clara cell function in the lungs, Cre recombinase was inserted into exon 1 of the CCSP, generating two novel mouse models, CCSP(Cre-Neo) and CCSP(Cre). These two models differ only by the inclusion of the neomycin resistance gene. These mice were bred to the R26R reporter mouse to investigate the tissue and cell specificity of Cre-mediated recombination. The efficiency of Cre recombination in the CCSP(Cre) mouse model was higher than in the CCSP(Cre-Neo) mouse model. Recombination was detected at D 4.5 in CCSP(Cre-Neo)/R26R mice and at D 0.5 in CCSP(Cre)/R26R mice. The CCSP(Cre-Neo) and CCSP(Cre) mouse models provide valuable tools for the ablation of genes in the postnatal mouse Clara cells.
File Format	HTM / HTML
ISSN	1526954X
e-ISSN	1526968X
DOI	10.1002/dvg.20396
Journal	genesis
Issue Number	6
Volume Number	46
Language	English
Publisher	Wiley-Liss
Publisher Date	2008-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Genetics Discipline Developmental Biology Discipline Embryology Integrases Genetics Recombination, Genetic Respiratory Mucosa Cytology Metabolism Uteroglobin Animals Crosses, Genetic Epithelial Cells Exons Gene Targeting Genes, Reporter Genetic Engineering Lung Mice Mice, Transgenic Models, Genetic Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Medicine Endocrinology

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Cre-mediated recombination in mouse Clara cells.

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