NDLI: Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sadate-Ngatchou, Patricia I. Payne, Christopher J. Dearth, Andrea T. Braun, Robert E.
Description	Author Affiliation: Sadate-Ngatchou PI ( Department of Genome Sciences, University of Washington School of Medicine, Seattle, Washington.)
Abstract	We have generated a transgenic mouse line,Tg(Stra8-cre)1Reb (Stra8-cre), which expresses improved Cre recombinase under the control of a 1.4 Kb promoter region of the germ cell-specific stimulated by retinoic acid gene 8 (Stra8). cre is expressed only in males beginning at postnatal day (P)3 in early-stage spermatogonia and is detected through preleptotene-stage spermatocytes. To further define when cre becomes active, we crossed Stra8-cre males with Tg(ACTB-Bgeo/GFP)21Lbe (Z/EG) reporter females and compared the expression of enhanced green fluorescent protein (EGFP) with the protein encoded by the zinc finger and BTB domain containing 16 (Zbtb16) gene, PLZF-a marker for undifferentiated spermatogonia. Co-expression of EGFP is observed in the majority of PLZF+ cells. We also tested recombination efficiency by mating Stra8-cre;Z/EG males and females with wild-type mice and examining EGFP expression in the offspring. Recombination is detected in >95% of Z/EG+ pups born to Stra8-cre;Z/EG fathers but in none of the offspring born to transgenic mothers, a verification that cre is not functional in females. The postnatal, premeiotic, male germ cell-specific activity of Stra8-cre makes this mouse line a unique resource to study testicular germ cell development.
File Format	HTM / HTML
ISSN	1526954X
e-ISSN	1526968X
DOI	10.1002/dvg.20437
Journal	genesis
Issue Number	12
Volume Number	46
Language	English
Publisher	Wiley-Liss
Publisher Date	2008-12-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Genetics Discipline Developmental Biology Discipline Embryology Integrases Metabolism Promoter Regions, Genetic Spermatocytes Growth & Development Spermatogenesis Genetics Adaptor Proteins, Signal Transducing Animals Cell Differentiation Cells, Cultured Embryo, Mammalian Genes, Reporter Mice Mice, Transgenic Proteins Recombination, Genetic
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Medicine Endocrinology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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