NDLI: Identification of side population cells in mouse primordial germ cells and prenatal testis.

Please wait, while we are loading the content...

Identification of side population cells in mouse primordial germ cells and prenatal testis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Scaldaferri, Maria-Lucia Fera, Stefania Grisanti, Laura Sanchez, Massimo Stefanini, Mario De Felici, Massimo Vicini, Elena
Description	Country affiliation: Italy Author Affiliation: Scaldaferri ML ( Department of Public Health and Cell Biology, University of Rome Tor Vergata, Rome, Italy.)
Abstract	In mammals, the stem cells of spermatogenesis are derived from an embryonic cell population called primordial germ cells (PGCs). Spermatogonial stem cells displaying the 'side population' (SP) phenotype have been identified in the immature and adult mouse testis, but noting is known about the expression of the SP phenotype during prenatal development of germ cells. The SP phenotype, defined as the ability of cells to efflux fluorescent dyes such as Hoechst, is common to several stem/progenitor cell types. In the present study, we analyzed and characterized the Hoechst SP via cytofluorimetric analysis of disaggregated gonads at different time points during embryonic development in mice. To directly test the hypothesis that the SP phenotype is a feature of germ cell lineage, experiments were performed on transgenic animals expressing enhanced green fluorescent protein (EGFP) under the control of the Oct4 promoter, to identify early germ cells up to PGCs. We found that prenatal gonads contain a fraction of SP cells at each stage analyzed, and the percentage of cells in the SP fraction decreases as development proceeds. Surprisingly, more than 50% of the PGCs displayed the SP phenotype at 11.5 dpc (days post coitum). The percentage of germ cells with the SP phenotype decreased steadily with development, to less than 1% at 18.5 dpc. Cytofluorimetric analysis along with immunocytochemistry performed on sorted cells indicated that the SP fraction of prenatal gonads, as in the adult testis, was heterogeneous, being composed of both somatic and germ cells. Both cell types expressed the ABC transporters Abcg2, Abcb1a, Abcb1b and Abcc1. These findings provide evidence that the SP phenotype is a common feature of PGCs and identifies a subpopulation of fetal testis cells including prospermatogonia whose differentiation fate remains to be investigated.
File Format	HTM / HTML
ISSN	02146282
e-ISSN	16963547
Journal	The International Journal of Developmental Biology
Issue Number	2
Volume Number	55
Language	English
Publisher	University of the Basque Country Press
Publisher Date	2011-01-01
Publisher Place	Spain
Access Restriction	Open
Subject Keyword	Discipline Embryology Discipline Biology Germ Cells Cytology Side-population Cells Testis Atp-binding Cassette Transporters Biosynthesis Genetics Animals Cell Differentiation Cell Lineage Flow Cytometry Metabolism Mice Mice, Transgenic Octamer Transcription Factor-3 Polymerase Chain Reaction Promoter Regions, Genetic Spermatogenesis Spermatogonia Embryology Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Embryology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in