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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mimura, Sumiyo Kimura, Naohiro Hirata, Mitsuhi Tateyama, Daiki Hayashida, Midori Umezawa, Akihiro Kohara, Arihiro Nikawa, Hiroki Okamoto, Tetsuji Furue, Miho K. |
| Description | Country affiliation: Japan Author Affiliation: Mimura S ( JCRB Cell Bank, Laboratory of Cell Cultures, Department of Disease Bioresources, National Institute of Biomedical Innovation, Osaka, Japan.) |
| Abstract | Human bone marrow-derived mesenchymal stem cells (hMSCs) are potential cellular sources of therapeutic stem cells as they have the ability to proliferate and differentiate into a wide array of mesenchymal cell types such as osteoblasts, chondroblasts and adipocytes. hMSCs have been used clinically to treat patients with graft vs. host disease, osteogenesis imperfect, or alveolar cleft, suggesting that transplantation of hMSCs is comparatively safe as a stem cell-based therapy. However, conventional culture medium for hMSCs contains fetal bovine serum (FBS). In the present study, we developed a growth factor-defined, serum-free medium for culturing hMSCs. Under these conditions, TGF-beta1 promoted proliferation of hMSCs. The expanded hMSC population expressed the human pluripotency markers SSEA-3, -4, NANOG, OCT3/4 and SOX2. Furthermore, double positive cells for SSEA-3 and a mesenchymal cell marker, CD105, were detected in the population. The potential to differentiate into osteoblasts and adipocytes was confirmed. This work provides a useful tool to understand the basic biological properties of hMSCs in culture. |
| File Format | HTM / HTML |
| ISSN | 02146282 |
| e-ISSN | 16963547 |
| Journal | The International Journal of Developmental Biology |
| Issue Number | 2 |
| Volume Number | 55 |
| Language | English |
| Publisher | University of the Basque Country Press |
| Publisher Date | 2011-01-01 |
| Publisher Place | Spain |
| Access Restriction | Open |
| Subject Keyword | Discipline Embryology Discipline Biology Cell Proliferation Intercellular Signaling Peptides And Proteins Pharmacology Mesenchymal Stromal Cells Drug Effects Metabolism Pluripotent Stem Cells Tissue Culture Techniques Antigens, Cd Biosynthesis Antigens, Tumor-associated, Carbohydrate Cell Differentiation Cells, Cultured Culture Media, Serum-free Homeodomain Proteins Octamer Transcription Factor-3 Receptors, Cell Surface Soxb1 Transcription Factors Stage-specific Embryonic Antigens Transforming Growth Factor Beta Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Embryology |
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