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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Aulenta, Federico Verdini, Roberta Zeppilli, Marco Zanaroli, Giulio Fava, Fabio Rossetti, Simona Majone, Mauro |
| Description | Author Affiliation: Aulenta F ( Water Research Institute, National Research Council (IRSA-CNR), Via Salaria km 29.300, 00015 Monterotondo, RM, Italy. Electronic address: aulenta@irsa.cnr.it.) |
| Abstract | This study investigated the feasibility of using a polarized graphite electrode as direct or indirect (via electrolytic oxygen generation) electron acceptor to stimulate the microbial oxidation of cis-dichloroethene (cis-DCE) in contaminated groundwater. A microbial culture was enriched in the anode chamber of a bioelectrochemical cell using a mixture of cis-DCE and ethene as substrates. The bioelectrochemical cell was operated by controlling the anode potential at +1.0 V or +1.5 V versus the standard hydrogen electrode (SHE). Enhanced cometabolic removal of cis-DCE, with ethene serving as the growth substrate, was observed in batch tests with the anode polarized at +1.5 V versus SHE. At this potential, (chloro)ethenes removal was probably sustained by molecular oxygen generated at the anode from water oxidation. Conversely, negligible anaerobic degradation was observed at +1.0 V versus SHE (a potential which does not allow oxygen generation), hence suggesting that molecular oxygen is needed to initiate (chloro)ethene degradation. PCR-DGGE analysis of the microbial culture followed by band sequencing and phylogenetic analysis evidenced the selective enrichment of a Bacillus species, providing a strong indication that this microorganism was responsible for cis-DCE and ethene degradation. |
| File Format | HTM / HTML |
| ISSN | 18716784 |
| Issue Number | 6 |
| Volume Number | 30 |
| e-ISSN | 18764347 |
| Journal | New Biotechnology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-09-25 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Discipline Molecular Biology Bacillus Metabolism Dichloroethylenes Ethylenes Graphite Growth & Development Biodegradation, Environmental Chemistry Electrodes Oxidation-reduction Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Bioengineering Biotechnology |
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