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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Botta, Anna Lucia Santacecilia, Alessandra Ercole, Claudia Cacchio, Paola Del Gallo, Maddalena |
| Description | Country affiliation: Italy Author Affiliation: Botta AL ( Department MeSVA, Environmental Microbiology Laboratory, University of L'Aquila, Coppito 67010 L'Aquila, Italy.) |
| Abstract | Four bacteria selected on the basis of their capability of fixing atmospheric nitrogen, stimulating plant-growth, and protecting the host plant from pathogens - Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, Burkholderia ambifaria - were inoculated on tomato seeds either singularly, in couple and in a four bacteria mixer. Aim of this research was to evaluate: (1) effect of single and mixed cultures on the inoculated plant - plant growth, dry weight, root length and surface, number of leaves, among others; (2) colonization and interactions of the bacteria inside the host plant; (3) localization inside the host of single bacterial strains marked with the gusA reporter gene. The results obtained indicate that all selected microbial strains have colonized Lycopersicon esculentum but in a different way, depending on the single species. A. brasilense, G. diazotrophicus inoculated in vitro singularly and together were the best plant colonizers. In vivo essays, instead, B. ambifaria and the four-bacteria mixer gave the best results. It was possible to localize both A. brasilense and H. seropedicae inside the plant by the gusA reporter gene. The bacterial strains occur along the root axis from the apical zone until to the basal stem, on the shoot from the base up to the leaves. The four bacteria actively colonize tomato seeds and establish an endophytic community inside the plant. This review gives new information about colonization processes, in particular how bacteria interact with plants and whether they are likely to establish themselves in the plant environment after field application as biofertilizers or biocontrol agents. |
| File Format | HTM / HTML |
| ISSN | 18716784 |
| Issue Number | 6 |
| Journal | New Biotechnology |
| Volume Number | 30 |
| e-ISSN | 18764347 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-09-25 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Discipline Molecular Biology Bacteria Growth & Development Lycopersicon Esculentum Plant Stems Seeds Symbiosis Metabolism Microbiology Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Bioengineering Biotechnology |
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