NDLI: Pseudooutbreak of Acinetobacter spp. Bacteriuria Confirmed by 16S rRNA Gene Sequence Analysis

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Pseudooutbreak of Acinetobacter spp. Bacteriuria Confirmed by 16S rRNA Gene Sequence Analysis

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Pseudooutbreak of Acinetobacter spp. Bacteriuria Confirmed by 16S rRNA Gene Sequence Analysis

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, S. Y. Kim, J. Y. Kang, J. H. Park, S. Y. Lee, H. S. Park, Y. S. Seo, Y. H. Cho, Y. K.
Description	Responsible library: WPRO
Abstract	BACKGROUND: Acinetobacter spp. is increasingly implicated in hospital-acquired infections. We experienced a pseudooutbreak of Bordetella bronchiseptica bacteriuria identified with biochemical tests, that was later identified as Acinetobacter spp. by using 16S rRNA gene sequence analysis.MATERIALS AND METHODS: Five in-ward patients were found to have B. bronchiseptica bacteriuria without symptoms of urinary tract infection between September 23 and 26 of 2005. We conducted pulsed field gel electrophoresis (PFGE) of the bacteria and epidemiological investigation of this pseudooutbreak. In addition, 16S rRNA gene sequence analysis was performed for the verification of the strains.RESULTS: All 5 isolates were identified as B. bronchiseptica with similar antibiogram by VITEK system. There was no evidence of any symptom or sign of urinary tract infection. The source of this pseudooutbreak was not detected even after performing environmental culture and interviews with healthcare workers. We could not get the appropriate results from the first PFGE with XbaI restriction enzyme. B. bronchiseptica is an unusual organism in human so we conducted 16S rRNA gene sequence analysis for verification. The analysis of 16S rRNA gene sequence with 5 isolates demonstrated 99-100% similarity to a sequence of Acinetobacter spp. (AU1523). According to the results of 16S rRNA gene sequence analysis, we performed the second PFGE with SmaI restriction enzyme, which showed indistinguishable pattern among the all 5 isolates. CONCLUSION: This investigation suggests that the combined method of 16s rRNA gene sequence analysis and PFGE would be helpful for investigation of outbreak caused by unusual organisms
File Format	HTM / HTML
ISSN	20932340
e-ISSN	20926448
Journal	Infection and Chemotherapy
Issue Number	4
Volume Number	39
Language	English Korean
Publisher	Korean Society for Chemotherapy
Publisher Date	2007-01-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Communicable Diseases Discipline Therapeutics Rna, Ribosomal Genes Analysis Of Substances Electrophoresis, Gel, Pulsed-field Acinetobacter
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Pharmacology (medical)

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