NDLI: Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE)

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Molecular Epidemiologic Analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) Producing Escherichia coli Using Infrequent-Restriction-Site Polymerase Chain Reaction (IRS-PCR) with Comparison by Pulsed-Field Gel Electrophoresis (PFGE)

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Byun, Ji Hyun Yoo, Jin Hong Park, Chulmin Lee, Dong Gun Park, Sun Hee Choi, Su Mi Choi, Jung Hyun Kim, Si Hyun Kwon, Jae Cheol
Description	Responsible library: WPRO
Abstract	BACKGROUND: We evaluated the ability of infrequent restriction site-polymerase chain reaction (IRS-PCR) to perform molecular epidemiologic analysis of Community-Onset Extended Spectrum Beta-Lactamase (ESBL) producing Escherichia coli, and also assessed the use of PFGE as an alternative method. MATERIALS AND METHODS: IRS-PCR assay was performed using combinations of adaptors for XbaI and HhaI restriction sites on clinical isolates of E. coli (n=51). We compared the discriminatory power, quality and efficiency of IRS-PCR to PFGE. RESULTS: In E. coli, PFGE discriminated 39 (76.4%) and IRS-PCR discerned 41 (80.3%) of the total 51 strains. It took much less time to complete IRS-PCR (one day) than PFGE (at least 4 days). CONCLUSIONS: IRS-PCR is a more sensitive and rapid alternative to PFGE for molecular epidemiologic analysis of E. coli.
File Format	HTM / HTML
ISSN	20932340
e-ISSN	20926448
Journal	Infection and Chemotherapy
Issue Number	1
Volume Number	44
Language	English Korean
Publisher	Korean Society for Chemotherapy
Publisher Date	2012-01-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Communicable Diseases Discipline Therapeutics
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Pharmacology (medical)

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