NDLI: Carbon isotope ratio mass spectrometry for detection of endogenous steroid use: a testing strategy.

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Carbon isotope ratio mass spectrometry for detection of endogenous steroid use: a testing strategy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ahrens, Brian D. Butch, Anthony W.
Description	Country affiliation: United States Author Affiliation: Ahrens BD ( UCLA Olympic Analytical Laboratory, Department of Pathology & Laboratory Medicine, Geffen School of Medicine, Reagan UCLA Medical Center, Los Angeles, CA 90095-7418, USA. abutch@mednet.ucla.edu)
Abstract	Isotope ratio mass spectrometry (IRMS) testing is performed to determine if an atypical steroid profile is due to administration of an endogenous steroid. Androsterone (Andro) and etiocholanolone (Etio), and/or the androstanediols (5 - and 5ß-androstane-3 ,17ß-diol) are typically analyzed by IRMS to determine the (13) C/(12) C ratio. The ratios of these target compounds are compared to the (13) C/(12) C ratio of an endogenous reference compound (ERC) such as 5ß-pregnane-3 ,20 -diol (Pdiol). Concentrations of Andro and Etio are high so (13) C/(12) C ratios can easily be measured in most urine samples. Despite the potentially improved sensitivity of the androstanediols for detecting the use of some testosterone formulations, additional processing steps are often required that increase labour costs and turnaround times. Since this can be problematic when performing large numbers of IRMS measurements, we established thresholds for Andro and Etio that can be used to determine the need for additional androstanediol testing. Using these criteria, 105 out of 2639 urine samples exceeded the Andro and/or Etio thresholds, with 52 of these samples being positive based on Andro and Etio IRMS testing alone. The remaining 53 urine samples had androstanediol IRMS testing performed and 3 samples were positive based on the androstanediol results. A similar strategy was used to establish a threshold for Pdiol to identify athletes with relatively (13) C-depleted values so that an alternative ERC can be used to confirm or establish a true endogenous reference value. Adoption of a similar strategy by other laboratories can significantly reduce IRMS sample processing and analysis times, thereby increasing testing capacity.
File Format	HTM / HTML
ISSN	19427603
Issue Number	7
Volume Number	5
e-ISSN	19427611
Journal	Drug Testing and Analysis
Language	English
Publisher	Wiley
Publisher Date	2013-07-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Pharmacology Athletes Carbon Isotopes Doping In Sports Mass Spectrometry Methods Steroids Urine Substance Abuse Detection Androsterone Etiocholanolone Humans Standards Journal Article
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Environmental Chemistry Analytical Chemistry Pharmaceutical Science

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The application of carbon isotope ratio mass spectrometry to doping control.

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Certification of steroid carbon isotope ratios in a freeze-dried human urine reference material.

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Validity of stable isotope data in doping control: perspectives and proposals.

Complementary stable carbon isotope ratio and amount of substance measurements in sports anti-doping.

Isotope ratio mass spectrometry - history and terminology in brief.

Carbon isotope ratio mass spectrometry for detection of endogenous steroid use: a testing strategy.

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