NDLI: Zinc availability during germline development impacts embryo viability in Caenorhabditis elegans.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mendoza, Adelita D. Woodruff, Teresa K. Wignall, Sarah M. O'Halloran, Thomas V.
Description	Country affiliation: United States Author Affiliation: Mendoza AD ( Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA); Woodruff TK ( Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA); Wignall SM ( Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA. Electronic address: s-wignall@northwestern.edu.); O'Halloran TV ( Department of Molecular Biosciences, Northwestern University, Evanston, IL 60208, USA)
Abstract	Zinc is an essential metal that serves as a cofactor in a variety of cellular processes, including meiotic maturation. Cellular control of zinc uptake, availability and efflux is closely linked to meiotic progression in rodent and primate reproduction where large fluctuations in zinc levels are critical at several steps in the oocyte-to-embryo transition. Despite these well-documented roles of zinc fluxes during meiosis, only a few of the genes encoding key zinc receptors, membrane-spanning transporters, and downstream signaling pathway factors have been identified to date. Furthermore, little is known about analogous roles for zinc fluxes in the context of a whole organism. Here, we evaluate whether zinc availability regulates germline development and oocyte viability in the nematode Caenorhabditis elegans, an experimentally flexible model organism. We find that similar to mammals, mild zinc limitation in C. elegans profoundly impacts the reproductive axis: the brood size is significantly reduced under conditions of zinc limitation where other physiological functions are not perturbed. Zinc limitation in this organism has a more pronounced impact on oocytes than sperm and this leads to the decrease in viable embryo production. Moreover, acute zinc limitation of isolated zygotes prevents extrusion of the second polar body during meiosis and leads to aneuploid embryos. Thus, the zinc-dependent steps in C. elegans gametogenesis roughly parallel those described in meiotic-to-mitotic transitions in mammals.
File Format	HTM / HTML
ISSN	15320456
Volume Number	191
Journal	Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Language	English
Publisher	Elsevier
Publisher Date	2017-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Toxicology Discipline Pharmacology Caenorhabditis Elegans Metabolism Gametogenesis Oocytes Spermatozoa Zinc Aneuploidy Animals Animals, Genetically Modified Drug Effects Enzymology Cell Survival Chelating Agents Pharmacology Chromosome Segregation Embryo, Nonmammalian Pathology Ethylamines Female Genotype Male Phenotype Pyridines Time Factors Deficiency Journal Article Video-audio Media
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology Health, Toxicology and Mutagenesis Medicine Aquatic Science Biochemistry Toxicology Animal Science and Zoology

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