| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Singh, Arjun Taylor, Larry E. Vander Wall, Todd A. Linger, Jeffrey Himmel, Michael E. Podkaminer, Kara Adney, William S. Decker, Stephen R. |
| Description |
Country affiliation: United States Author Affiliation: Singh A ( National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Taylor LE ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Vander Wall TA ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Linger J ( National Bioenergy Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Himmel ME ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Podkaminer K ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Adney WS ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States.); Decker SR ( Biosciences Center, National Renewable Energy Laboratory, 15013 Denver West Parkway, Golden, CO 80401, United States. Electronic address: Steve.decker@nrel.gov.) |
| Abstract |
Hypocrea jecorina, the sexual teleomorph of Trichoderma reesei, has long been favored as an industrial cellulase producer, first utilizing its native cellulase system and later augmented by the introduction of heterologous enzymatic activities or improved variants of native enzymes. Expression of heterologous proteins in H. jecorina was once considered difficult when the target was an improved variant of a native cellulase. Developments over the past nearly 30 years have produced strains, vectors, and selection mechanisms that have continued to simplify and streamline heterologous protein expression in this fungus. More recent developments in fungal molecular biology have pointed the way toward a fundamental transformation in the ease and efficiency of heterologous protein expression in this important industrial host. Here, 1) we provide a historical perspective on advances in H. jecorina molecular biology, 2) outline host strain engineering, transformation, selection, and expression strategies, 3) detail potential pitfalls when working with this organism, and 4) provide consolidated examples of successful cellulase expression outcomes from our laboratory. |
| File Format |
HTM / HTML |
| ISSN |
07349750 |
| Issue Number |
1 |
| Volume Number |
33 |
| e-ISSN |
18731899 |
| Journal |
Biotechnology Advances |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-01-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Biotechnology Cellulase Metabolism Fungal Proteins Hypocrea Industrial Microbiology Trichoderma Genetics Drug Resistance, Fungal Genes, Fungal Genetic Loci Phylogeny Promoter Regions, Genetic Recombinant Proteins Journal Article Research Support, U.s. Gov't, Non-p.h.s. Review |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Bioengineering Applied Microbiology and Biotechnology Biotechnology |
