NDLI: Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh.

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Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh.

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Comparison of three different methods for effective introduction of platelet-rich plasma on PLGA woven mesh.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Ji-Hye Nam, Jinwoo Kim, Hee Joong Yoo, Jeong Joon
Description	Author Affiliation: Lee JH ( Department of Orthopaedic Surgery, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Korea.)
Abstract	For successful tissue regeneration, effective cell delivery to defect site is very important. Various types of polymer biomaterials have been developed and applied for effective cell delivery. PLGA (poly lactic-co-glycolic acid), a synthetic polymer, is a commercially available and FDA approved material. Platelet-rich plasma (PRP) is an autologous growth factor cocktail containing various growth factors including PDGF, TGFß-1 and BMPs, and has shown positive effects on cell behaviors. We hypothesized that PRP pretreatment on PLGA mesh using different methods would cause different patterns of platelet adhesion and stages which would modulate cell adhesion and proliferation on the PLGA mesh. In this study, we pretreated PRP on PLGA using three different methods including simple dripping (SD), dynamic oscillation (DO) and centrifugation (CE), then observed the amount of adhered platelets and their activation stage distribution. The highest amount of platelets was observed on CE mesh and calcium treated CE mesh. Moreover, calcium addition after PRP coating triggered dramatic activation of platelets which showed large and flat morphologies of platelets with rich fibrin networks. Human chondrocytes (hCs) and human bone marrow stromal cells (hBMSCs) were next cultured on PRP-pretreated PLGA meshes using different preparation methods. CE mesh showed a significant increase in the initial cell adhesion of hCs and proliferation of hBMSCs compared with SD and DO meshes. The results demonstrated that the centrifugation method can be considered as a promising coating method to introduce PRP on PLGA polymeric material which could improve cell-material interaction using a simple method.
File Format	HTM / HTML
ISSN	17486041
e-ISSN	1748605X
Journal	Biomedical Materials
Issue Number	2
Volume Number	10
Language	English
Publisher	IOP Publishing
Publisher Date	2015-03-11
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Biomedical Engineering Biocompatible Materials Lactic Acid Platelet-rich Plasma Cytology Polyglycolic Acid Tissue Engineering Blood Platelets Physiology Cell Adhesion Cell Proliferation Cells, Cultured Centrifugation Chondrocytes Coated Materials, Biocompatible Materials Testing Mesenchymal Stromal Cells Microscopy, Electron, Scanning Platelet Adhesiveness Regeneration Tissue Scaffolds Chemistry Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Chemistry Business and International Management Mechanics of Materials Biomaterials Bioengineering Biomedical Engineering

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