NDLI: Escherichia coli exposure inhibits exocytic SNARE-mediated membrane fusion in mast cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wesolowski, Jordan Paumet, Fabienne
Description	Country affiliation: United States Author Affiliation: Wesolowski J ( Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA, USA.)
Abstract	Mast cells orchestrate the allergic response through the release of proinflammatory mediators, which is driven by the fusion of cytoplasmic secretory granules with the plasma membrane. During this process, SNARE proteins including Syntaxin4, SNAP23 and VAMP8 play a key role. Following stimulation, the kinase IKKß interacts with and phosphorylates the t-SNARE SNAP23. Phosphorylated SNAP23 then associates with Syntaxin4 and the v-SNARE VAMP8 to form a ternary SNARE complex, which drives membrane fusion and mediator release. Interestingly, mast cell degranulation is impaired following exposure to bacteria such as Escherichia coli. However, the molecular mechanism(s) by which this occurs is unknown. Here, we show that E. coli exposure rapidly and additively inhibits degranulation in the RBL-2H3 rat mast cell line. Following co-culture with E. coli, the interaction between IKKß and SNAP23 is disrupted, resulting in the hypophosphorylation of SNAP23. Subsequent formation of the ternary SNARE complex between SNAP23, Syntaxin4 and VAMP8 is strongly reduced. Collectively, these results demonstrate that E. coli exposure inhibits the formation of VAMP8-containing exocytic SNARE complexes and thus the release of VAMP8-dependent granules by interfering with SNAP23 phosphorylation.
File Format	HTM / HTML
ISSN	13989219
e-ISSN	16000854
DOI	10.1111/tra.12159
Journal	Traffic
Issue Number	5
Volume Number	15
Language	English
Publisher	Wiley
Publisher Date	2014-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Physiology Escherichia Coli Metabolism Mast Cells Physiology Membrane Fusion Snare Proteins Animals Cell Line Coculture Techniques I-kappa B Kinase Membrane Microdomains Membrane Proteins Phosphorylation Protein Binding Qa-snare Proteins R-snare Proteins Vesicular Transport Proteins Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Structural Biology Molecular Biology Biochemistry

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