NDLI: Acute fasting before conception affects metabolic and endocrine status without impacting follicle and oocyte development and embryo gene expression in the rabbit.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	García-García, R. M. Rebollar, P. G. Arias-Álvarez, M. Sakr, O. G. Bermejo-Álvarez, P. Brecchia, G. Gutierrez-Adan, A. Zerani, M. Boiti, C. Lorenzo, P. L.
Description	Country affiliation: Spain Author Affiliation: García-García RM ( Dpto. Fisiología (Fisiología Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, s/n, Madrid 28040, Spain. rosa.garcia@vet.ucm.es)
Abstract	Food deprivation affects female reproduction. The goal of the present study was to elucidate in the rabbit model the effects of acute energy restriction on ovarian function (follicle development, atresia rate and in vitro oocyte maturation) and embryonic development and gene expression of some candidate genes. Serum metabolic parameters (non-esterified fatty acids (NEFA), triglycerides, glucose, insulin and leptin concentrations) and endocrine markers (oestradiol-17ß and progesterone concentrations) were also studied. A control group of nulliparous does fed ad libitum and a 72-h fasted group were used. At the end of the nutritional treatment, the ovaries of half of the animals were retrieved while the other animals were re-fed and artificially inseminated to recover embryos at 84 h after insemination, during the luteal phase. At the end of fasting, increased serum NEFA and decreased leptin concentrations were observed in the fasted group, but no differences appeared in serum steroid concentrations, follicle population and atresia rate or nuclear and cytoplasmic oocyte maturation. In the luteal phase, insulin concentrations increased notably in the fasted group. The number of recovered embryos per female and the speed of embryo development were reduced in the food-deprived group. Acute fasting altered both metabolic and endocrine markers and embryo development, but follicle and oocyte development and embryo gene expression were not affected.
File Format	HTM / HTML
ISSN	10313613
Issue Number	6
Volume Number	23
e-ISSN	14485990
Journal	Reproduction, Fertility and Development
Language	English
Publisher	CSIRO Publishing
Publisher Date	2011-01-01
Publisher Place	Australia
Access Restriction	Subscribed
Subject Keyword	Discipline Reproductive Medicine Endocrine System Physiology Energy Metabolism Fasting Gene Expression Regulation, Developmental Maternal-fetal Exchange Oocytes Growth & Development Ovarian Follicle Analysis Of Variance Animals Blood Glucose Analysis Body Composition Dna Primers Genetics Embryo, Mammalian Estradiol Blood Fatty Acids Female Gene Expression Profiling In Situ Nick-end Labeling Insulin Leptin Microscopy, Confocal Pregnancy Progesterone Rabbits Reverse Transcriptase Polymerase Chain Reaction Triglycerides Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology Molecular Biology Animal Science and Zoology Endocrinology Reproductive Medicine Biotechnology

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