NDLI: Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ledgard, A. M. Meier, S. Peterson, A. J.
Description	Country affiliation: New Zealand Author Affiliation: Ledgard AM ( AgResearch, Ruakura Research Centre, East Street, Hamilton 3240, New Zealand. anita.ledgard@agresearch.co.nz)
Abstract	During previous investigations, the capacity of the cow to secrete prostaglandin in response to oxytocin has been linked with pregnancy outcome. The objective of the present study was to evaluate the predictive value of prostaglandin release to identify groups of cows as potentially superior (SR, low prostaglandin release) or inferior (IR, high prostaglandin release) for pregnancy outcome and to utilise these cows to investigate factors that contribute to optimum uterine conditions for early pregnancy. Animals were synchronised and received an in vitro-derived blastocyst on Day 7 post-oestrus. Tissues (trophoblast and endometrial) and uterine luminal fluid (ULF) were recovered 10 days later. Pregnancy rates were 94 and 78% for SR and IR cows, respectively. Of the pregnant SR cows, 69% had larged conceptuses (>24 cm) in contrast to 43% IR of cows. IR cows with small conceptuses (<12 cm) had significantly lower mean Day 3 and 5 post-oestrous progesterone concentrations than cows with large conceptuses. The expression of factors involved in the prostaglandin pathway, pregnancy and conceptus development were analysed via quantitative RT-PCR and Western blot analysis. Investigation of 16 endometrial gene transcripts indicated no differences between IR and SR cows except for osteopontin expression which, in uteri with large conceptuses, was 2-fold greater in SR than IR cows (P=0.02). There was greater expression of CTGF, OXTR, PGES, PGHS2 and UTMP mRNA in uteri of SR and IR cows that had large compared with small conceptuses (P<0.05). More IFNT protein was recovered in SR compared with IR ULF (P<0.03). SR cows with large conceptuses had less TIMP2 and legumain protein in their gravid, compared with their non-gravid horns (P≤0.02) whereas IR cows did not. The predictive value of prostaglandin release in response to oxytocin challenge does not appear to be an effective indicator of subsequent pregnancy rates in cows. Differences between the two groups appear to be associated with subtle differences in progesterone and uterine protein concentrations that may be related to differences in conceptus size.
File Format	HTM / HTML
ISSN	10313613
Issue Number	6
Volume Number	23
e-ISSN	14485990
Journal	Reproduction, Fertility and Development
Language	English
Publisher	CSIRO Publishing
Publisher Date	2011-01-01
Publisher Place	Australia
Access Restriction	Subscribed
Subject Keyword	Discipline Reproductive Medicine Cattle Embryo, Mammalian Physiology Pregnancy Outcome Veterinary Pregnancy, Animal Prostaglandins Metabolism Uterus Animals Blotting, Western Body Size Breeding Methods Endometrium Female Gene Expression Profiling Osteopontin Oxytocin Pregnancy Real-time Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology Molecular Biology Animal Science and Zoology Endocrinology Reproductive Medicine Biotechnology

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