NDLI: A dispensable role for P450(scc) in the overproduction of aldosterone in aldosterone-producing adenoma and idiopathic hyperaldosteronism in patients with primary aldosteronism.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fang, Yujiang Zhao, Lei Zang, Meifu Chen, Songsen Yan, Feng Di, Xu Duren, Alicia
Description	Country affiliation: United States Author Affiliation: Fang Y ( Department of Internal Medicine, University of Missouri, Columbia, MO 65211, USA. fangy@health.missouri.edu)
Abstract	Our previous study suggests that cytochrome P-450 carbon 17 -hydroxylase/17,20-lyase (P450(c17 )) correlated with the overproduction of aldosterone in aldosterone-producing adenoma (APA) and idiopathic hyperaldosteronism (IHA) in patients with primary aldosteronism. To further investigate if cytochrome P-450 cholesterol side-chain cleavage enzyme (P450(scc)) contributes to the overproduction of aldosterone in APA and IHA and if its mRNA expression differs in APA and IHA in patients with primary aldosteronism, we studied the expression of P450(scc) mRNA in APA and idiopathic hyperplastic nodules. Total RNA was extracted from APA of eight patients diagnosed as APA, idiopathic hyperplastic nodules of four patients diagnosed as IHA, seven normal adrenal glands and one normal muscle tissue. P450(scc) mRNA was examined by Northern blot analysis. No significant difference in P450(scc) mRNA was found among normal adrenal gland, APA or idiopathic hyperplastic nodules (P > 0.05). These results suggest that P450(scc) contributes little to the overproduction of aldosterone in APA and IHA and cannot be considered as a marker to differentiate between them in patients with primary aldosteronism.
File Format	HTM / HTML
ISSN	12194956
Issue Number	4
Volume Number	16
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2010-12-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Adrenocortical Adenoma Metabolism Aldosterone Biosynthesis Cholesterol Side-chain Cleavage Enzyme Hyperaldosteronism Enzymology Adult Blotting, Northern Genetics Female Humans Male Rna, Messenger Retrospective Studies Young Adult Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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