NDLI: Involvement of c-Ski oncoprotein in carcinogenesis of cholangiocacinoma induced by Opisthorchis viverrini and N-nitrosodimethylamine.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Boonmars, Thidarut Wu, Zhiliang Boonjaruspinyo, Sirintip Puapairoj, Anucha Kaewsamut, Butsara Nagano, Isao Pinlaor, Somchai Yongvanit, Puangrat Wonkchalee, Orasa Juasook, Amornrat Sudsarn, Pakkayanee Srisawangwong, Tuanchai
Description	Country affiliation: Thailand Author Affiliation: Boonmars T ( Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. bthida@kku.ac.th)
Abstract	Opisthorchiasis is the major public health problem in the endemic areas of Thailand and Laos because Opisthorchis viverrini infection causes serious hepatobiliary diseases including CCA. The molecular mechanism of the CCA carcinogenesis induced by the infection remains obscure. To reveal the potential genes and signaling pathways to involve in the carcinogenesis, the present study investigated the expression of c-Ski, an oncogene, and two TGF-ß signaling pathway relative genes, TGF-ß and Smad4, during the development of CCA induced by O. viverrini infection in hamster model, and in human opisthorchiasis associated CCA. The results showed that the expression of c-Ski gene was greatly up-regulated during the carcinogenesis of CCA in hamster model. The overexpression of c-Ski was confirmed by immunohistological staining result which showed the increased expression of c-Ski protein in cytoplasm of the epithelial lining of hepatic bile ducts. Moreover, the immunohistological staining of the specimens of human opisthorchiasis associated CCA revealed the up-regulated expression of c-Ski and Smad4 proteins in the cytoplasm of the epithelial lining of hepatic bile ducts and stomal fibrosis respectively. The expression of TGF-ß and Smad4 were up-regulated, which expression kinetics was time-dependent of CCA development. These results suggest that c-Ski is likely involved in the carcinogenesis of CCA induced by O. viverrini infection through regulating TGF-ß signaling pathway.
File Format	HTM / HTML
ISSN	12194956
Issue Number	2
Volume Number	17
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2011-06-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Bile Duct Neoplasms Genetics Bile Ducts, Intrahepatic Pathology Cell Transformation, Neoplastic Cholangiocarcinoma Dna-binding Proteins Metabolism Opisthorchiasis Complications Opisthorchis Physiology Proto-oncogene Proteins Animals Chemically Induced Carcinogens Toxicity Cricetinae Dimethylnitrosamine Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Male Mesocricetus Reverse Transcriptase Polymerase Chain Reaction Smad4 Protein Biosynthesis Transforming Growth Factor Beta Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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