NDLI: MicroRNA-335 acts as a candidate tumor suppressor in prostate cancer.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Xiong, Si-wei Lin, Tian-xin Xu, Ke-wei Dong, Wen Ling, Xiao-hui Jiang, Fu-neng Chen, Guo Zhong, Wei-de Huang, Jian
Description	Country affiliation: China Author Affiliation: Xiong SW ( Department of Urology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China.)
Abstract	MicroRNA-335 (miR-335) acts as a tumor suppressor or a tumor promoter in different human malignancies. However, the involvement of miR-335 in prostate cancer (PCa) is still unclear. The purpose of this study was to investigate the functional and clinical significance of miR-335 in PCa. miR-335 expression in 3 PCa cell lines (LNCaP/DU145/PC3) and in 20 clinical PCa tissues were detected by real-time quantitative reverse transcriptase-PCR compared with corresponding controls. The function of miR-335 was investigated for cell proliferation, invasion and migration in PCa cells transfected with agents containing EGFP-miR-335 expression vector. Additionally, miR-335 expression in 104 clinical PCa tissues was detected by in situ hybridization. Its assocaitions with clinicopathological features and prognosis in patients with PCa were also determined. miR-335 was significantly down-regulated in PCa cell lines than in the normal prostate cell line (P < 0.01). With the similar results in vitro, the reduced expression of miR-335 was also found in human PCa tissues comparing with paired adjacent benign prostate tissues (P < 0.05). Moreover, the increased expression of miR-335 suppressed cell proliferation, invasion and migration of PCa cell lines in vitro. Turning to its clinical significance, the low expression of miR-335 was significantly associated with high Gleason Score (P = 0.04), advanced clinical stage (P = 0.04), and positive metastasis (P = 0.02), but not with prognosis in PCa patients. Our data demonstrated for the first time the inhibitory effect of miR-335 on cell proliferation and invasion for PCa cells. The loss of this microRNA might be associated with clinical progression of PCa patients.
File Format	HTM / HTML
ISSN	12194956
Issue Number	3
Volume Number	19
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2013-07-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Micrornas Metabolism Prostatic Neoplasms Tumor Suppressor Proteins Adult Aged Analysis Of Variance Cell Growth Processes Physiology Cell Line, Tumor Cell Movement Down-regulation Humans Kaplan-meier Estimate Male Biosynthesis Genetics Middle Aged Neoplasm Invasiveness Prognosis Pathology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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