NDLI: Metal retention in human transferrin: Consequences of solvent composition in analytical sample preparation methods

Please wait, while we are loading the content...

(99m)Tc-labeling of ciprofloxacin and nitrofuryl thiosemicarbazone using fac-[(99m)Tc(CO)3(H2O)3] core: evaluation of their efficacy as infection imaging agents.

Simple and rapid quantification of gadolinium in urine and blood plasma samples by means of total reflection X-ray fluorescence (TXRF).

Combined application of a laser ablation-ICP-MS assay for screening and ESI-FTICR-MS for identification of a Cd-binding protein in Spinacia oleracea L. after exposure to Cd.

Speciation of oxaliplatin adducts with DNA nucleotides.

Disorders associated with systemic or local iron overload: from pathophysiology to clinical practice.

Metal retention in human transferrin: Consequences of solvent composition in analytical sample preparation methods

Inhibitory effect of platinum and ruthenium bipyridyl complexes on porcine pancreatic phospholipase A2.

The regulatory role of heme in neurons.

LC– and CZE – ICP-MS approaches for the in vivo analysis of the anticancer drug candidate sodium trans-[tetrachloridobis(1H-indazole)ruthenate(III)] (KP1339) in mouse plasma

Absorption and retention characteristics of selenium in dorsal root ganglion neurons.

Reactions of metallodrugs with proteins: selective binding of phosphane-based platinum(II) dichlorides to horse heart cytochrome c probed by ESI MS coupled to enzymatic cleavage.

A novel nickel responsive MerR-like regulator, NimR, from Haemophilus influenzae.

Year: 2011 Volume: 3 Issue: 9

Year: 2011 Volume: 3 Issue: 8

Year: 2011 Volume: 3 Issue: 7

Year: 2011 Volume: 3 Issue: 6

Year: 2011 Volume: 3 Issue: 5

Year: 2011 Volume: 3 Issue: 4

Year: 2011 Volume: 3 Issue: 3

Year: 2011 Volume: 3 Issue: 2

Year: 2011 Volume: 3 Issue: 1

Year: 2010 Volume: 2

Year: 2009 Volume: 1

Metal retention in human transferrin: Consequences of solvent composition in analytical sample preparation methods

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Quarles, C. Derrick Randunu, K. Manoj Brumaghim, Julia L. Marcus, R. Kenneth
Description	Country affiliation: United States Author Affiliation: Quarles CD ( Department of Chemistry, Biosystems Research Complex, Clemson University, Clemson, South Carolina 29634-0973, USA.)
Abstract	The analysis of metal-binding proteins requires careful sample manipulation to ensure that the metal– protein complex remains in its native state and the metal retention is preserved during sample preparation or analysis. Chemical analysis for the metal content in proteins typically involves some type of liquid chromatography /electrophoresis separation step coupled with an atomic (i.e., inductively coupled plasma-optical emission spectroscopy or -mass spectrometry ) or molecular (i.e., electrospray ionization- mass spectrometry ) analysis step that requires altered- solvent introduction techniques. UV-VIS absorbance is employed here to monitor the iron content in human holo-transferrin (Tf) under various solvent conditions, changing polarity, pH, ionic strength, and the ionic and hydrophobic environment of the protein . Iron loading percentages (i.e. 100% loading equates to 2 $Fe^{3+} : 1$ Tf) were quantitatively determined to evaluate the effect of solvent composition on the retention of $Fe^{3+}$ in Tf. Maximum retention of $Fe^{3+}$ was found in buffered (20 mM Tris ) solutions (96 ± 1%). Exposure to organic solvents and deionized H $_{2}O$ caused release of ∼23–36% of the $Fe^{3+}$ from the binding pocket(s) at physiological pH (7.4). Salt concentrations similar to separation conditions used for ion exchange had little to no effect on $Fe^{3+}$ retention in holo-Tf. Unsurprisingly, changes in ionic strength caused by additions of guanidine HCl (0–10 M) to holo-Tf resulted in unfolding of the protein and loss of $Fe^{3+}$ from Tf; however, denaturing and metal loss was found not to be an instantaneous process for additions of 1–5 M guanidinium to Tf. In contrast, complete denaturing and loss of $Fe^{3+}$ was instantaneous with ≥6 M additions of guanidinium, and denaturing and loss of iron from Tf occurred in parallel proportions. Changes to the hydrophobicity of Tf (via addition of 0–14 M urea ) had less effect on denaturing and release of $Fe^{3+}$ from the Tf binding pocket compared to changes in ionic strength.
File Format	HTM / HTML
ISSN	17565901
Issue Number	10
Volume Number	3
e-ISSN	1756591X
Journal	Metallomics
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Iron Metabolism Transferrin Chromatography, Reverse-phase Circular Dichroism Humans Protein Binding Protein Denaturation Spectrophotometry, Ultraviolet Chemistry Journal Article
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mammalian MT1 and MT2 metallothioneins differ in their metal binding abilities.

Reference measurement procedures for the iron saturation in human transferrin based on IDMS and Raman scattering

Titanium preferential binding sites in human serum transferrin at physiological concentrations

The importance of metal ions for the formation and isolation of insulin-like growth factor-binding protein 3–transferrin (IGFBP-3–Tf) complexes, and the analysis of their physiological involvement

Raman spectroscopy a promising technique for investigations of metallothioneins

Metal ions binding to NAD-glycohydrolase from the venom of Agkistrodon acutus: Regulation of multicatalytic activity

Hijacking transferrin bound iron: protein-receptor interactions essential for iron transport in N. gonorrhoeae

Monitoring protein aggregation during thermal unfolding in circular dichroism experiments.

Hijacking transferrin bound iron: protein–receptor interactions involved in iron transport in N. gonorrhoeae

Metal retention in human transferrin: Consequences of solvent composition in analytical sample preparation methods

Similar Documents

Mammalian MT1 and MT2 metallothioneins differ in their metal binding abilities.

Reference measurement procedures for the iron saturation in human transferrin based on IDMS and Raman scattering

Titanium preferential binding sites in human serum transferrin at physiological concentrations

The importance of metal ions for the formation and isolation of insulin-like growth factor-binding protein 3–transferrin (IGFBP-3–Tf) complexes, and the analysis of their physiological involvement

Raman spectroscopy a promising technique for investigations of metallothioneins

Metal ions binding to NAD-glycohydrolase from the venom of Agkistrodon acutus: Regulation of multicatalytic activity

Hijacking transferrin bound iron: protein-receptor interactions essential for iron transport in N. gonorrhoeae

Monitoring protein aggregation during thermal unfolding in circular dichroism experiments.

Hijacking transferrin bound iron: protein–receptor interactions involved in iron transport in N. gonorrhoeae

Metal retention in human transferrin: Consequences of solvent composition in analytical sample preparation methods