NDLI: Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.

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Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Simons, Keryn L. Thomas, Susan M. Anderson, Peter A.
Description	Country affiliation: Australia Author Affiliation: Simons KL ( School of Biological Sciences, Flinders University, Adelaide, SA 5001, Australia. Keryn.Simons@flinders.edu.au)
Abstract	Nucleotide excision repair (NER) rectifies a variety of chemically and structurally distinct DNA lesions. The current model of NER is based upon the enteric bacterium Escherichia coli and there is scarce information about how other bacterial species respond to, and correct, DNA damage. Here we report the isolation and functional analysis of the uvrA and uvrB genes from Vibrio natriegens, a naturally occurring marine bacterium. Genetic studies were completed to assess the repair capabilities of V. natriegens uvrA and uvrB in E. coli uvrA and uvrB mutants. In addition to the genetic studies, transcriptional fusions between the luciferase gene and the 5' regulatory regions of uvrA and uvrB gene of V. natriegens and E. coli were constructed. Luminescent measurements from E. coli transformed with these constructs showed that whilst the response to UV irradiation of either E. coli or V. natriegens uvrA regulatory sequences was similar, both the rate and induction of luminescence detected from the uvrB regulatory regions differed.
File Format	HTM / HTML
ISSN	12258873
Issue Number	5
Volume Number	48
e-ISSN	19763794
Journal	Journal of Microbiology
Language	English
Publisher	Springer
Publisher Date	2010-10-01
Publisher Place	Korea (South)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Bacterial Proteins Genetics Metabolism Dna Repair Dna-binding Proteins Gene Expression Regulation, Bacterial Vibrio Enzymology Amino Acid Sequence Artificial Gene Fusion Base Sequence Cloning, Molecular Escherichia Coli Genes, Reporter Genetic Complementation Test Luciferases Luminescent Measurements Molecular Sequence Data Promoter Regions, Genetic Suppression, Genetic Ultraviolet Rays Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids

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Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.

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Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids

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Identification of Vibrio natriegens uvrA and uvrB genes and analysis of gene regulation using transcriptional reporter plasmids.