NDLI: Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r-MCP43) of giant freshwater prawn, M. rosenbergii (de Man) for immunological diagnostic methods.

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Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r-MCP43) of giant freshwater prawn, M. rosenbergii (de Man) for immunological diagnostic methods.

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Production of recombinant capsid protein of Macrobrachium rosenbergii nodavirus (r-MCP43) of giant freshwater prawn, M. rosenbergii (de Man) for immunological diagnostic methods.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Farook, M. A. Madan, N. Taju, G. Majeed, S. Abdul Nambi, K. S. N. Raj, N. Sundar Vimal, S. Hameed, A. S. Sahul
Description	Country affiliation: India Author Affiliation: Farook MA ( OIE Reference Laboratory for WTD, Department of Zoology, C. Abdul Hakeem College, Melvisharam, India.)
Abstract	White tail disease (WTD) caused by Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) is a serious problem in prawn hatcheries. The gene for capsid protein of MrNV (MCP43) was cloned into pRSET B expression vector. The MCP43 protein was expressed as a protein with a 6-histidine tag in Escherichia coli GJ1158 with NaCl induction. This recombinant protein, which was used to raise the antiserum in rabbits, recognized capsid protein in different WTD-infected post-larvae and adult prawn. Various immunological methods such as Western blot, dot blot and ELISA techniques were employed to detect MrNV in infected samples using the antiserum raised against recombinant MCP43 of MrNV. The dot blot assay using anti-rMCP43 was found to be capable of detecting MrNV in WTD-infected post-larvae as early as at 24 h post-infection. The antiserum raised against r-MCP43 could detect the MrNV in the infected samples at the level of 100 pg of total protein. The capsid protein of MrNV estimated by ELISA using anti-rMCP43 and pure r-MCP43 as a standard was found to increase gradually during the course of infection from 24 h p.i. to moribund stage. The results of immunological diagnostic methods employed in this study were compared with that of RT-PCR to test the efficiency of antiserum raised against r-MCP43 for the detection of MrNV. The Western blot, dot blot and ELISA detected all MrNV-positive coded samples as detected by RT-PCR.
File Format	HTM / HTML
ISSN	01407775
Issue Number	8
Volume Number	37
e-ISSN	13652761
Journal	Journal of Fish Diseases
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2014-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Zoology Capsid Proteins Immunology Metabolism Immunoassay Methods Nodaviridae Isolation & Purification Palaemonidae Virology Animals Gene Expression Regulation, Viral Physiology Host-pathogen Interactions Larva Life Cycle Stages Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Veterinary Aquatic Science

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