NDLI: Biocompatibility evaluation of dicalcium phosphate/calcium sulfate/poly (amino acid) composite for orthopedic tissue engineering in vitro and in vivo.

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Biocompatibility evaluation of dicalcium phosphate/calcium sulfate/poly (amino acid) composite for orthopedic tissue engineering in vitro and in vivo.

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Biocompatibility evaluation of dicalcium phosphate/calcium sulfate/poly (amino acid) composite for orthopedic tissue engineering in vitro and in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Peng Liu, Pengzheng Peng, Haitao Luo, Xiaoman Yuan, Huipin Zhang, Juncai Yan, Yonggang
Description	Country affiliation: China Author Affiliation: Wang P ( a College of Physical Science and Technology , Sichuan University , Chengdu , China.); Liu P ( b Sichuan Guona Technology Co., LTD , Chengdu , China.); Peng H ( a College of Physical Science and Technology , Sichuan University , Chengdu , China.); Luo X ( c Xpand Biotechnology BV , Bilthoven , The Netherlands.); Yuan H ( a College of Physical Science and Technology , Sichuan University , Chengdu , China.); Zhang J ( c Xpand Biotechnology BV , Bilthoven , The Netherlands.); Yan Y ( d College of Chemical Engineering and Chemistry , Xianyang Normal University , Xianyang , China.)
Abstract	In vitro cytocompatibility of ternary biocomposite of dicalcium phosphate (DCP) and calcium sulfate (CS) containing 40 wt% poly (amino acid) (PAA) was evaluated using L929 ï¬ broblasts and MG-63 osteoblast-like cells. Thereafter, the biocompatibility of biocomposite in vivo was investigated using an implantation in muscle and bone model. In vitro L929 and MG-63 cell culture experiments showed that the composite and PAA polymer were noncytotoxic and allowed cells to adhere and proliferate. The scanning electron microscope (SEM) confirmed that two kinds of cells maintained their phenotype on all of samples surfaces. Moreover, the DCP/CS/PAA composite showed higher cellular viability than that of PAA; meanwhile, the cell proliferation and ALP activity were much higher when DCP/CS had added into PAA. After implanted in muscle of rabbits for 12 weeks, the histological evaluation indicated that the composite exhibited excellent biocompatibility and no inflammatory responses were found. When implanted into bone defects of femoral condyle of rabbits, the composite was combined directly with the host bone tissue without fibrous capsule tissue, which shown good biocompatibility and osteoconductivity. Thus, this novel composite may have potential application in the clinical setting.
File Format	HTM / HTML
ISSN	09205063
Issue Number	11
Journal	Journal of Biomaterials Science, Polymer Edition
Volume Number	27
e-ISSN	15685624
Language	English
Publisher	Taylor & Francis
Publisher Date	2016-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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