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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sivamaruthi, Bhagavathi Sundaram Ganguli, Abhijit Kumar, Mukesh Bhaviya, Sheker Pandian, Shunmugiah Karutha Balamurugan, Krishnaswamy |
| Description | Country affiliation: India Author Affiliation: Sivamaruthi BS ( Department of Biotechnology, Alagappa University, Karaikudi, India.) |
| Abstract | Cronobacter sakazakii is occasionally associated with food-borne illness seen in neonates and infants with weakened immune system. It can cause meningitis, local necrotizing enterocolitis and systemic bacteremia leading to infant mortality rates upto 33–80%. With the aim of investigating whether C. sakazakii is also a pathogen of the model organism C. elegans, we have performed killing assays and monitored the mortality of host fed with pathogen. C. elegans fed with C. sakazakii die over the course of several days, as a consequence of an accumulation of bacteria in the host intestine. Further, the rate of C. sakazakii mediated infection in C. elegans depends on the accumulation of the bacterial load inside the host. C. sakazakii killed C. elegans with an $LT_{50}$ (time for half to die) of 134 ± 2.8 h in liquid assay conditions, whereas the mortality of C. elegans infected with C. sakazakii was less pronounced during solid assays. We found that 24 h of C. sakazakii infection is enough to cause gametogenesis defects and increased cell damage in intestinal tract of host. To monitor the immune regulations during C. sakazakii infection in C. elegans at molecular level, total RNA was isolated and few candidate genes (lys-7, clec-60 and clec-87) were kinetically analyzed by using the semi-quantitative RT-PCR. The level of expression of lys-7, clec-60 and clec-87 mRNAs isolated from C. elegans infected with C. sakazakii was significantly higher when compared to C. elegans exposed to E. coli OP50 control. This is the first report in which physiological changes and an induction of host immunity mediated antimicrobial genes by C. sakazakii are shown in C. elegans. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| File Format | HTM / HTML |
| ISSN | 0233111X |
| Issue Number | 5 |
| Volume Number | 51 |
| e-ISSN | 15214028 |
| Journal | Journal of Basic Microbiology |
| Language | English |
| Publisher | Wiley-VCH Verlag |
| Publisher Date | 2011-10-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Caenorhabditis Elegans Cronobacter Sakazakii Pathogenicity Disease Models, Animal Enterobacteriaceae Infections Microbiology Animals Genetics Immunology Gene Expression Regulation Intestines Lectins, C-type Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Applied Microbiology and Biotechnology |
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