NDLI: Discovery of common urinary biomarkers for hepatotoxicity induced by carbon tetrachloride, acetaminophen and methotrexate by mass spectrometry-based metabolomics.

Please wait, while we are loading the content...

Year: 2017 Volume: 37

Year: 2016 Volume: 36

Year: 2015 Volume: 35

Year: 2014 Volume: 34

Year: 2013 Volume: 33

Year: 2012 Volume: 32

Year: 2012 Volume: 32 Issue: 12

Year: 2012 Volume: 32 Issue: 11

Year: 2012 Volume: 32 Issue: 10

Year: 2012 Volume: 32 Issue: 9

Year: 2012 Volume: 32 Issue: 8

Year: 2012 Volume: 32 Issue: 7

Comparative study on toxic effects induced by oral or intravascular administration of commonly used disinfectants and surfactants in rats.

Subchronic inhalation toxicity of iron oxide (magnetite, $Fe_{3}O_{4})$ in rats: pulmonary toxicity is determined by the particle kinetics typical of poorly soluble particles

Atrial natriuretic peptides in Han Wistar, Sprague-Dawley and spontaneously hypertensive rats.

Discovery of common urinary biomarkers for hepatotoxicity induced by carbon tetrachloride, acetaminophen and methotrexate by mass spectrometry-based metabolomics.

Chlorpyrifos exposure reduces reproductive capacity owing to a damaging effect on gametogenesis in the nematode Caenorhabditis elegans.

Year: 2012 Volume: 32 Issue: 6

Year: 2012 Volume: 32 Issue: 5

Year: 2012 Volume: 32 Issue: 4

Year: 2012 Volume: 32 Issue: 3

Year: 2012 Volume: 32 Issue: 2

Year: 2012 Volume: 32 Issue: 1

Year: 2011 Volume: 31

Year: 2010 Volume: 30

Year: 2009 Volume: 29

Year: 2008 Volume: 28

Discovery of common urinary biomarkers for hepatotoxicity induced by carbon tetrachloride, acetaminophen and methotrexate by mass spectrometry-based metabolomics.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kumar, Bhowmik Salil Chung, Bong Chul Kwon, Oh-Seung Jung, Byung Hwa
Description	Author Affiliation: Kumar BS ( Biomolecular Functional Research Center, Korea Institute of Science and Technology, Cheongryang, Seoul, Republic of Korea.)
Abstract	Liver toxicity represents an important healthcare issue because it causes significant morbidity and mortality and can be difficult to predict before symptoms appear owing to drug therapy or exposure to toxicants. Using metabolomic techniques, we discovered common biomarkers for the prediction of hepatotoxicity in rat urine using mass spectrometry. For this purpose, liver toxicity was induced by 5 days of oral administration of carbon tetrachloride (1 ml kg(-1) per day), acetaminophen (1000 mg kg(-1) per day) and methotrexate (50 mg kg(-1) per day). Serum levels of alkaline phosphatase aspartate aminotransferase, alanine aminotransferase and histopathology in liver tissue were then checked to demonstrate liver toxicity. Global metabolic profiling with UPLC-TOF-MS (ultraperformance liquid chromatography-mass spectrometry), multivariate analysis (partial least square-discriminant analysis, hierarchical analysis) and database searching were performed to discover common biomarkers for liver toxicity induced by these three compounds. Urinary concentrations of the newly discovered biomarkers were then quantified to confirm them as biomarkers of hepatotoxicity with targeted metabolic profiling using GC (gas chromatography)-MS and CE (capillary electrophoresis)-MS. In the results, steroids, amino acids and bile acids were metabolically changed between the control and drug-treated groups in global metabolic profiling; 11ß-hydroxyandrosterone, epiandrosterone, estrone, 11-dehydrocorticosterone, glycine, alanine, valine, leucine, dl-ornithine, 3-methylhistidine, cholic acid and lithocholic acid were selected as liver toxicity biomarkers after performing targeted metabolic profiling. In conclusion, we discovered metabolite biomarkers belonging to three different metabolic pathways to check for liver toxicity with mass spectrometry from a metabolomics study that could be used to evaluate hepatotoxicity induced by drugs or other toxic compounds.
File Format	HTM / HTML
ISSN	0260437X
Issue Number	7
Volume Number	32
e-ISSN	10991263
Journal	Journal of Applied Toxicology
Language	English
Publisher	Wiley
Publisher Date	2012-07-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Toxicology Acetaminophen Toxicity Biological Markers Urine Carbon Tetrachloride Drug-induced Liver Injury Metabolism Mass Spectrometry Methods Metabolomics Methotrexate Alanine Transaminase Blood Alkaline Phosphatase Animals Aspartate Aminotransferases Bile Acids And Salts Physiopathology Chromatography, Liquid Discriminant Analysis Liver Male Metabolome Multivariate Analysis Rats Rats, Sprague-dawley Reproducibility Of Results Steroids Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Toxicology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in