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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lu, Yuan Zhang, Chong Lai, Qiheng Zhao, Hongxin Xing, Xin-Hui |
| Description | Country affiliation: China Author Affiliation: Lu Y ( Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China.) |
| Abstract | Effects of different microaerophilic conditions on cell growth, glucose consumption, hydrogen production and cellular metabolism of wild Enterobacter aerogenes strain and polyphosphate kinase (PPK) overexpressing strain were systematically studied in this paper, using NaH(2)PO(4) as the phosphate sources. Under different microaerophilic conditions, PPK-overexpressing strain showed better cell growth, glucose consumption and hydrogen production than the wild strain. In the presence of limited oxygen (2.1%) and by PPK overexpression, the hydrogen production per liter of culture, the hydrogen production per cell and the hydrogen yield per mol of glucose increased by 20.1%, 12.3% and 10.8%, respectively, compared with the wild strain under strict anaerobic conditions. Metabolic analysis showed that the increase of the total hydrogen yield was attributed to the improvement of NADH pathway. The result of more reductive cellular oxidation state balance also further demonstrated that, under proper initial microaerophilic conditions and by PPK overexpression, the cell could adjust the cellular redox states and make more energy flow into hydrogen production pathways. |
| File Format | HTM / HTML |
| ISSN | 01410229 |
| Issue Number | 2 |
| Volume Number | 48 |
| e-ISSN | 18790909 |
| Journal | Enzyme and Microbial Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-02-08 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Biochemistry Discipline Biotechnology Enterobacter Aerogenes Enzymology Gene Expression Regulation, Enzymologic Hydrogen Metabolism Phosphotransferases (phosphate Group Acceptor) Anaerobiosis Genetics Growth & Development Gene Expression Regulation, Bacterial Glucose Industrial Microbiology Methods Oxidation-reduction Oxygen Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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