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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Niraula, Narayan Prasad Kanth, Bashistha Kumar Sohng, Jae Kyung Oh, Tae-Jin |
| Description | Author Affiliation: Niraula NP ( Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, Sun Moon University, #100 Kalsan-ri, Tangjeong-myeon, Asansi, Chungnam 336-708, Republic of Korea.) |
| Abstract | Cytochrome P450 CYP107AJ1, which was isolated from Streptomyces peucetius and showed high homology with peroxygenases, catalyzed a dealkylation reaction with hydrogen peroxide to provide electrons, protons and oxygen, evading the requirement for a supporting redox protein. Preliminary investigation of its transcriptional level in S. peucetius showed significant expression. Homology modeling and subsequent docking with 7-ethoxycoumarin yielded a reasonable docked structure. cyp107AJ1 cloned into pET28a(+) was expressed in Escherichia coli, and soluble protein was subjected to column-chromatographic purification in order to carry out enzyme assays with 7-ethoxycoumarin. HPLC analysis of the extracted product, corresponding to its LC/MS analysis, showed the dealkylated 7-ethoxycoumarin, which was further established by subsequent GC/MS spectral analysis. We suggest that CYP107AJ1 bypassed the requirement for NAD(P)H and redox partners for generating novel analogues. |
| File Format | HTM / HTML |
| ISSN | 01410229 |
| Issue Number | 2 |
| Volume Number | 48 |
| e-ISSN | 18790909 |
| Journal | Enzyme and Microbial Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-02-08 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Biochemistry Discipline Biotechnology Biotechnology Methods Coumarins Metabolism Cytochrome P-450 Enzyme System Chemistry Hydrogen Peroxide Pharmacology Streptomyces Enzymology Amino Acid Sequence Genetics Dealkylation Drug Effects Escherichia Coli Models, Molecular Molecular Sequence Data Recombinant Proteins Sequence Alignment Sequence Homology, Amino Acid Structural Homology, Protein Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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