NDLI: Use of voltage clamp fluorimetry in understanding potassium channel gating: a review of Shaker fluorescence data.

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Use of voltage clamp fluorimetry in understanding potassium channel gating: a review of Shaker fluorescence data.

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Use of voltage clamp fluorimetry in understanding potassium channel gating: a review of Shaker fluorescence data.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Horne, A. J. Fedida, D.
Description	Country affiliation: Canada Author Affiliation: Horne AJ ( Department of Cellular and Physiological Sciences, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.)
Abstract	Voltage clamp fluorimetry (VCF) utilizes fluorescent probes that covalently bind to cysteine residues introduced into proteins and emit light as a function of their environment. Measurement of this emitted light during membrane depolarization reveals changes in the emission level as the environment of the labelled residue changes. This allows for the correlation of channel gating events with movement of specific protein moieties, at nanosecond time resolution. Since the pioneering use of this technique to investigate Shaker potassium channel activation movements, VCF has become an invaluable technique used to understand ion channel gating. This review summarizes the theory and some of the data on the application of the VCF technique. Although its usage has expanded beyond voltage-gated potassium channels and VCF is now used in a number of other voltage- and ligand-gated channels, we will focus on studies conducted in Shaker potassium channels, and what they have told us about channel activation and inactivation gating.
File Format	HTM / HTML
ISSN	00084212
e-ISSN	12057541
Journal	Canadian Journal of Physiology and Pharmacology
Issue Number	6
Volume Number	87
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2009-06-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Physiology Discipline Pharmacology Fluorometry Ion Channel Gating Physiology Shaker Superfamily Of Potassium Channels Animals Fluorescent Dyes Models, Molecular Mutation Patch-clamp Techniques Protein Conformation Rhodamines Chemistry Genetics
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Pharmacology

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