NDLI: Assessing the utility of three TaqMan probes for the diagnosis of tuberculosis and resistance to rifampin and isoniazid in Veracruz, México.

Please wait, while we are loading the content...

Characterizing the structural diversity of a bacterial community associated with filter materials in recirculating aquaculture systems of Scortum barcoo.

Structure of bacterial communities in diverse freshwater habitats.

Assessing the utility of three TaqMan probes for the diagnosis of tuberculosis and resistance to rifampin and isoniazid in Veracruz, México.

Identification of envelope protein orf10 of channel catfish herpesvirus.

Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems.

Cell surface properties of two differently virulent strains of Acinetobacter baumannii isolated from a patient.

Disruption of genes for the enhanced biosynthesis of -ketoglutarate in Corynebacterium glutamicum.

A liquid chromatography - mass spectrometry method to measure ¹³C-isotope enrichment for DNA stable-isotope probing.

Genetic variability in arbuscular mycorrhizal fungi compatibility supports the selection of durum wheat genotypes for enhancing soil ecological services and cropping systems in Canada.

Fisher scientific award lecture - the capsular polysaccharides of Group B Streptococcus and Streptococcus suis differently modulate bacterial interactions with dendritic cells.

Method for the validation of intraspecific crosses of Saccharomyces cerevisiae strains by minisatellite analysis.

Change in antimicrobial susceptibility of Escherichia coli urinary tract isolates at a single institution over a period of 10 years.

Heterologous expression of bacterial nitric oxide synthase gene: a potential biological method to control biofilm development in the environment.

Year: 2012 Volume: 58 Issue: 2

Year: 2012 Volume: 58 Issue: 1

Assessing the utility of three TaqMan probes for the diagnosis of tuberculosis and resistance to rifampin and isoniazid in Veracruz, México.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zenteno-Cuevas, Roberto Cuevas-Cordoba, Betzaida Enciso, Antonio Enciso, Leonor Cuellar, Aremy
Spatial Coverage	Mexico
Description	Author Affiliation: Zenteno-Cuevas R ( Instituto de Salud Pública, Universidad Veracruzana, Av. Luis Castelazo Ayala s/n, A.P. 57, Col. Industrial Animas, Xalapa, Veracruz, 91190 México. robzencue@gmail.com)
Abstract	Mutations at codons 526 and 531 in the rpoB gene and at 315 in the katG gene are considered diagnostic markers for resistance to rifampin and isoniazid in tuberculosis. The aim of this study was to design and evaluate three TaqMan probes for the identification of these mutations in 138 respiratory samples positive for acid-fast bacilli, and 32 clinical isolates from a region with considerable levels of drug resistance. The specificities of the probes for the diagnosis of resistance to both drugs were 100%; however, the sensitivities were calculated to be 50% for isoniazid and 56% for rifampin. DNA sequencing of rpoB and katG; and the spoligotyping assay of the clinical isolates, confirmed the diversity of the mutations and the presence of 11 spoligotypes with a shared international type and eight unique spoligotypes. Analysis of the respiratory samples identified 22 (16%) as drug-resistant and 4 (3%) as multidrug-resistant tuberculosis. The diagnostic value of the TaqMan probes was compromised by the diversity of mutations found in the clinical isolates. This highlights the need for better understanding of the molecular mechanisms responsible for drug resistance prior to the use of molecular probes, especially in regions with significant levels of drug-resistant tuberculosis.
File Format	HTM / HTML
ISSN	00084166
e-ISSN	14803275
Journal	Canadian Journal of Microbiology
Issue Number	3
Volume Number	58
Language	English
Publisher	Canadian Science Publishing
Publisher Date	2012-03-01
Publisher Place	Canada
Access Restriction	Open
Subject Keyword	Discipline Microbiology Isoniazid Pharmacology Molecular Diagnostic Techniques Mycobacterium Tuberculosis Drug Effects Rifampin Tuberculosis, Multidrug-resistant Diagnosis Tuberculosis Antitubercular Agents Bacterial Proteins Genetics Catalase Dna-directed Rna Polymerases Genetic Variation Mexico Mutation Isolation & Purification Sensitivity And Specificity Sequence Analysis, Dna Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine Molecular Biology Immunology Microbiology Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genotyping and molecular characteristics of multidrug-resistant Mycobacterium tuberculosis isolates from China.

Molecular mechanism of rifampicin and isoniazid resistance in Mycobacterium tuberculosis from Bangladesh.

Rifampicin resistance in tuberculosis outbreak, London, England.

Defining multidrug-resistant tuberculosis: correlating GenoType MTBDRplus assay results with minimum inhibitory concentrations.

Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria.

Prevalence and risk factors of drug-resistant tuberculosis in border provinces of Iran.

Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolated from south-central in China.

Determinants of multidrug-resistant tuberculosis clusters, California, USA, 2004-2007.

Prevalence of mutations conferring resistance among multi- and extensively drug-resistant Mycobacterium tuberculosis isolates in China.

Assessing the utility of three TaqMan probes for the diagnosis of tuberculosis and resistance to rifampin and isoniazid in Veracruz, México.

Similar Documents

Genotyping and molecular characteristics of multidrug-resistant Mycobacterium tuberculosis isolates from China.

Molecular mechanism of rifampicin and isoniazid resistance in Mycobacterium tuberculosis from Bangladesh.

Rifampicin resistance in tuberculosis outbreak, London, England.

Defining multidrug-resistant tuberculosis: correlating GenoType MTBDRplus assay results with minimum inhibitory concentrations.

Systematic interpretation of molecular beacon polymerase chain reaction for identifying rpoB mutations in Mycobacterium tuberculosis isolates with mixed resistant and susceptible bacteria.

Prevalence and risk factors of drug-resistant tuberculosis in border provinces of Iran.

Molecular characterization of multidrug-resistant Mycobacterium tuberculosis isolated from south-central in China.

Determinants of multidrug-resistant tuberculosis clusters, California, USA, 2004-2007.

Prevalence of mutations conferring resistance among multi- and extensively drug-resistant Mycobacterium tuberculosis isolates in China.

Assessing the utility of three TaqMan probes for the diagnosis of tuberculosis and resistance to rifampin and isoniazid in Veracruz, México.