NDLI: Toxic effects of dexamethasone on mouse testicular germ cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Orazizadeh, M. Khorsandi, L. S. Hashemitabar, M.
Description	Country affiliation: Iran Author Affiliation: Orazizadeh M ( Cell and molecular research center, Faculty of Medicine, University of Medical Sciences, Ahwaz Jundi-Shapour, Iran.)
Abstract	Exposure to glucocorticoids (GCs) leads to numerous changes in various biological systems including the reproductive system. This work evaluated effects of dexamethasone (Dex), a widely used GC, on mouse testicular germ cells. Experimental groups (E1-E3) received one of the following treatments daily for 7 days: 4, 7 and 10 mg kg(-1) Dex respectively. Control groups were treated with equivalent volumes of saline. Testicular histopathology, morphometric analysis and deoxy-UTP-digoxigenin nick end labeling (TUNEL) assessment were performed for evaluation of the toxic effects of Dex and detection of the apoptotic cells. The results showed that Dex induces histopathological alterations such as epithelial vacuolisation, atrophy and reduction in testicular spermatozoid. Morphometrical data showed that Dex significantly reduced tubular diameter and epithelial height (P < 0.05). Johnsen's scoring also showed poor spermatogenesis in E2 and E3 groups (P < 0.05). Apoptotic index of germ cells was significantly increased in E2 (18.9% versus 1.76%, P < 0.01) and E3 (24.6 versus 1.76%, P < 0.001) groups. It is concluded that Dex acts as testicular toxicant and that further studies are needed to establish its mechanism of action upon spermatogenesis.
File Format	HTM / HTML
ISSN	03034569
Issue Number	4
Volume Number	42
e-ISSN	14390272
Journal	Andrologia
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-08-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Urology Discipline Reproductive Medicine Dexamethasone Toxicity Germ Cells Drug Effects Glucocorticoids Spermatozoa Animals Apoptosis In Situ Nick-end Labeling Male Mice Spermatogenesis Pathology Testis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Urology Endocrinology

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