NDLI: Effects of varicocele upon the expression of apoptosis-related proteins.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chang, F-W Sun, G-H Cheng, Y-Y Chen, I-C Chien, H-H Wu, G-J
Description	Country affiliation: Taiwan Author Affiliation: Chang FW ( Department of Obstetrics and Gynecology, Reproductive Medicine Center, Taipei, Taiwan.)
Abstract	Varicocele-associated apoptosis has been recognised as a cause of male infertility. Thus, we assessed the expression of somatic apoptosis-related proteins (the typical protein-dependent apoptosis markers) in ejaculated sperm plasma from both patients with varicocele and normal donors. We evaluated the relationships between certain apoptosis-related proteins and normal semen quality. Semen samples were obtained from 25 patients with varicocele and from 10 normal fertile controls. These samples were compared using computer-assisted semen analysis for motion parameters and manual analysis for morphology, and were also assayed for apoptosis-related protein activation including caspase-3, poly-ACP-ribose polymerase (PARP), the Bcl-2 family (Bcl-2, Bak) and p53 by means of immunoblot analysis. PARP, Bak and p53 were expressed substantially more in the sperm cells of the varicocele group when compared with the normal group (P < 0.05). The expression of caspase-3 and Bcl-2 did not appear to differ between these two study groups. An increased expression of PARP, Bak and p53 for varicocele-afflicted individuals indicated an increased participation by these agents in the regulating of apoptosis in the ejaculated semen from patients with varicocele, suggesting that certain protein-development apoptotic mechanisms might originate in the cytoplasmic droplet or within mitochondria of spermatocytes and then might function within the nucleus of the cell.
File Format	HTM / HTML
ISSN	03034569
Issue Number	4
Volume Number	42
e-ISSN	14390272
Journal	Andrologia
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2010-08-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Urology Discipline Reproductive Medicine Apoptosis Physiology Spermatozoa Metabolism Varicocele Physiopathology Adult Caspase 3 Biosynthesis Ejaculation Gene Expression Humans Infertility, Male Male Poly(adp-ribose) Polymerases Proto-oncogene Proteins C-bcl-2 Semen Analysis Tumor Suppressor Protein P53 Bcl-2 Homologous Antagonist-killer Protein Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Urology Endocrinology

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