NDLI: Combined effect of DHA and -tocopherol supplementation during bull semen cryopreservation on sperm characteristics and fatty acid composition.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nasiri, A. H. Towhidi, A. Zeinoaldini, S.
Description	Country affiliation: Iran Author Affiliation: Nasiri AH ( Department of Animal science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran.)
Abstract	The aim of this study was to investigate the effect of adding n-3 fatty acids (FA) and -tocopherol (VE) to semen extender on freezing ability and FA composition of bull sperm. Semen was collected from 10 Iranian Holstein bulls and was pooled. In the first experiment, semen was divided into 12 groups including 4 levels of n-3 FA (0, 0.1, 1, 10 ng ml(-1) ) and 3 levels of VE (0, 0.1, 0.2 mmol). The treatment of 0.2 mmol VE and 10 ng ml(-1) n-3 FA had the best post-thawed sperm characteristics (P < 0.01). In the second experiment, lipid composition of the latest treatment and control (without FA and VE) was determined. Adding n-3 FA increased docosahexaenoic acid (DHA) percentage before freezing and after thawing. The ratio of n-3 to n-6 before freezing was higher (P < 0.05) in treated group than in control, and this ratio in the fresh sperm was greater than in the post-thawed sperm (P = 0.1). Results suggested that adding DHA accompanied with an antioxidant to an extender could improve cryosurvival of bull sperm via altering membrane lipid composition.
File Format	HTM / HTML
ISSN	03034569
Issue Number	Suppl 1
Volume Number	44
e-ISSN	14390272
Journal	Andrologia
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2012-05-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Urology Discipline Reproductive Medicine Cryopreservation Docosahexaenoic Acids Administration & Dosage Fatty Acids Analysis Semen Preservation Spermatozoa Chemistry Alpha-tocopherol Animals Cattle Male Journal Article
Content Type	Text
Resource Type	Article
Subject	Urology Endocrinology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in