NDLI: CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.

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CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.

Year: 2009 Volume: 11 Issue: 3

Year: 2009 Volume: 11 Issue: 2

Year: 2009 Volume: 11 Issue: 1

Year: 2008 Volume: 10

CpG methylation analysis--current status of clinical assays and potential applications in molecular diagnostics: a report of the Association for Molecular Pathology.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sepulveda, Antonia R. Jones, Dan Ogino, Shuji Samowitz, Wade Gulley, Margaret L. Edwards, Robin Levenson, Victor Pratt, Victoria M. Yang, Bin Nafa, Khedoudja Yan, Liying Vitazka, Patrick
Description	Country affiliation: United States Author Affiliation: Sepulveda AR ( Methylation Working Group of the Association for Molecular Pathology Clinical Practice Committee, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.)
Abstract	Methylation of CpG islands in gene promoter regions is a major molecular mechanism of gene silencing and underlies both cancer development and progression. In molecular oncology, testing for the CpG methylation of tissue DNA has emerged as a clinically useful tool for tumor detection, outcome prediction, and treatment selection, as well as for assessing the efficacy of treatment with the use of demethylating agents and monitoring for tumor recurrence. In addition, because CpG methylation occurs early in pre-neoplastic tissues, methylation tests may be useful as markers of cancer risk in patients with either infectious or inflammatory conditions. The Methylation Working Group of the Clinical Practice Committee of the Association of Molecular Pathology has reviewed the current state of clinical testing in this area. We report here our summary of both the advantages and disadvantages of various methods, as well as the needs for standardization and reporting. We then conclude by summarizing the most promising areas for future clinical testing in cancer molecular diagnostics.
File Format	HTM / HTML
ISSN	15251578
e-ISSN	19437811
DOI	10.2353/jmoldx.2009.080125
Journal	The Journal of Molecular Diagnostics
Issue Number	4
Volume Number	11
Language	English
Publisher	Elsevier
Publisher Date	2009-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Tumor Markers, Biological Genetics Cpg Islands Dna Methylation Gene Expression Regulation, Neoplastic Neoplasms Diagnosis Antineoplastic Agents Therapeutic Use Dna Restriction Enzymes Metabolism Disease Progression Gene Expression Profiling Gene Silencing Molecular Sequence Data Classification Pathology Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic Reproducibility Of Results Sequence Analysis, Dna Sulfites Chemistry
Content Type	Text
Resource Type	Article
Subject	Molecular Medicine Pathology and Forensic Medicine

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