NDLI: Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli.

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Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Suzuki, Satoshi Matsumura, Norikazu Ohoka, Takeshi Sakuma, Shun Nakahata, Tomohiro Ishikawa, Masahide
Description	Country affiliation: Japan Author Affiliation: Suzuki S ( Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.); Matsumura N ( Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.); Ohoka T ( Department of Applied Chemistry, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.); Sakuma S ( Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.); Nakahata T ( Department of Applied Chemistry, Faculty of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan.); Ishikawa M ( Department of Materials Science and Engineering, Graduate School of Engineering, Saitama Institute of Technology, Fukaya 369-0293, Japan)
Abstract	Enzymes fixed on the electrode of biosensor are gradually inactivated and the electrode is discarded after using several times. In order to prepare the stable biosensor, we try to use a stable enzyme from extreme thermophilic bacteria, Thermus thermophilus HB8. It is very important that a stable enzyme from T. thermophilus HB8 is overproduced in Escherichia coli, for the purpose of enough supply of enzyme. Thereby, we determined the important sequence for overexpression of NADH oxidase (nox) gene from T. thermophilus HB8 in E. coli. As a result, it is revealed that ten nucleotides sequence, GAAATTAACT, in the upstream of start codon of nox gene was important for its overexpression in E. coli.
File Format	HTM / HTML
ISSN	10010742
Issue Number	Suppl 1
Volume Number	21
Journal	Journal of Environmental Sciences
Language	English
Publisher	Elsevier
Publisher Date	2009-01-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Health Escherichia Coli Genetics Multienzyme Complexes Nadh, Nadph Oxidoreductases Thermus Thermophilus Enzymology Base Sequence Electrophoresis, Polyacrylamide Gel Genes, Bacterial Genetic Vectors Metabolism Molecular Sequence Data Solubility Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Chemistry Medicine Environmental Engineering

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Overexpression of NADH oxidase gene from Deinococcus geothermalis in Escherichia coli.

Enzyme-catalyzed and binding reaction kinetics determined by titration calorimetry☆

Flexibility and enzyme activity of NADH oxidase from Thermus thermophilus in the presence of monovalent cations of Hofmeister series

Conformational plasticity surrounding the active site of NADH oxidase from Thermus thermophilus

Cloning and characterization of a thermostable H2O-forming NADH oxidase from Lactobacillus rhamnosus.

Improvement of exopolysaccharide production in Lactobacillus casei LC2W by overexpression of NADH oxidase gene.

Cloning, Expression and Characterization of Recombinant, NADH Oxidase from Giardia lamblia

Purification and properties of reconstitutively active and inactive adenosinetriphosphatase from Escherichia coli.

Structure of the phenylalnyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli

Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli.

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