NDLI: Proteasomes in human bronchoalveolar lavage fluid after burn and inhalation injury.

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Proteasomes in human bronchoalveolar lavage fluid after burn and inhalation injury.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Albright, Joslyn M. Romero, Jacqueline Saini, Vikas Sixt, Stephan U. Bird, Melanie D. Kovacs, Elizabeth J. Gamelli, Richard L. Peters, Jürgen Majetschak, Matthias
Description	Country affiliation: United States Author Affiliation: Albright JM ( Department of Surgery, Burn and Shock Trauma Institute, Loyola University Chicago Stritch School of Medicine, Maywood, Illinois 60153, USA.)
Abstract	The purpose of this study was to determine whether 26S proteasome is detectable in human bronchoalveolar lavage fluid (BALF) and whether burn and inhalation injury is accompanied by changes in BALF proteasome content or activity. BALF was obtained on hospital admission from 28 patients with burn and inhalation injury (controls: 10 healthy volunteers). Proteasome concentrations were quantified by enzyme-linked immunosorbent assay, and their native molecular mass was assessed by gel filtration. Proteasome peptidase activity was measured using a chymotryptic-like peptide substrate in combination with epoxomicin (specific proteasome inhibitor). BALF protein was increased in patients (P<.001) and correlated positively with the degree of inhalation injury. The 20S/26S proteasomes were detectable in all BALF by enzyme-linked immunosorbent assay. Gel filtration confirmed the presence of intact 20S and 26S proteasome that was stable without soluble ATP/Mg. In all BALF chymotryptic-like activity was detectable and could be inhibited with epoxomicin by 60 to 70% (P<.01). Absolute amounts of 20S/26S proteasomes and proteasome activity were increased in patients (P<.001 for all). The relative BALF composition after injury was characterized by increased concentrations of 20S proteasome/mg protein (P=.0034 vs volunteers), decreased concentrations of 26S proteasome/mg protein (P=.041 vs volunteers), and reduced specific proteasome activity (P=.044 vs volunteers). The 26S proteasome per milligram and specific proteasome activity were even further reduced in patients who developed ventilator-associated pneumonia (P=.045 and P=.03 vs patients without ventilator-associated pneumonia). This study supports the novel concept that extracellular proteasomes could play a pathophysiological role in the injured lung and suggests that insufficient proteasome function may increase susceptibility for pulmonary complications.
File Format	HTM / HTML
ISSN	1559047X
e-ISSN	15590488
DOI	10.1097/BCR.0b013e3181c07f37
Journal	Journal of Burn Care & Research
Issue Number	6
Volume Number	30
Language	English
Publisher	Lippincott Williams & Wilkins
Publisher Date	2009-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Traumatology Bronchoalveolar Lavage Fluid Chemistry Burns Enzymology Proteasome Endopeptidase Complex Metabolism Smoke Inhalation Injury Case-control Studies Chromatography, Gel Enzyme-linked Immunosorbent Assay Regression Analysis Statistics, Nonparametric Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Emergency Medicine Rehabilitation Surgery

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