Proteasomes in Human Bronchoalveolar Lavage Fluid After Burn and Inhalation Injury

Content Provider	Scilit
Author	Albright, Joslyn M. Romero, Jacqueline Saini, Vikas Sixt, Stephan U. Bird, Melanie D. Kovacs, Elizabeth J. Gamelli, Richard L. Peters, Jürgen Majetschak, Matthias
Copyright Year	2009
Description	Journal: Journal of Burn Care & Research The purpose of this study was to determine whether 26S proteasome is detectable in human bronchoalveolar lavage fluid (BALF) and whether burn and inhalation injury is accompanied by changes in BALF proteasome content or activity. BALF was obtained on hospital admission from 28 patients with burn and inhalation injury (controls: 10 healthy volunteers). Proteasome concentrations were quantified by enzyme-linked immonosorbent assay, and their native molecular mass was assessed by gel filtration. Proteasome peptidase activity was measured using a chymotryptic-like peptide substrate in combination with epoxomicin (specific proteasome inhibitor). BALF protein was increased in patients (P < .001) and correlated positively with the degree of inhalation injury. The 20S/26S proteasomes were detectable in all BALF by enzyme-linked immonosorbent assay. Gel filtration confirmed the presence of intact 20S and 26S proteasome that was stable without soluble $ATP/Mg^{2+}$. In all BALF chymotryptic-like activity was detectable and could be inhibited with epoxomicin by 60 to 70% (P < .01). Absolute amounts of 20S/26S proteasomes and proteasome activity were increased in patients (P < .001 for all). The relative BALF composition after injury was characterized by increased concentrations of 20S proteasome/mg protein (P = .0034 vs volunteers), decreased concentrations of 26S proteasome/mg protein (P = .041 vs volunteers), and reduced specific proteasome activity (P = .044 vs volunteers). The 26S proteasome per milligram and specific proteasome activity were even further reduced in patients who developed ventilator-associated pneumonia (P = .045 and P = .03 vs patients without ventilator-associated pneumonia). This study supports the novel concept that extracellular proteasomes could play a pathophysiological role in the injured lung and suggests that insufficient proteasome function may increase susceptibility for pulmonary complications.
Related Links	http://europepmc.org/articles/pmc3904667?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3904667/pdf https://academic.oup.com/jbcr/article-pdf/30/6/948/22408208/01253092-200911000-00005.pdf
Ending Page	956
Page Count	9
Starting Page	948
ISSN	1559047X
e-ISSN	15590488
DOI	10.1097/bcr.0b013e3181c07f37
Journal	Journal of Burn Care & Research
Issue Number	6
Volume Number	30
Language	English
Publisher	Ovid Technologies (Wolters Kluwer Health)
Publisher Date	2009-10-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Burn Care & Research Respiratory System Extracellular Proteasomes 26s Proteasome Inhalation Injury Protein Degradation Ventilator-associated Pneumonia
Content Type	Text
Resource Type	Article
Subject	Emergency Medicine Rehabilitation Surgery