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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Antunes, Sara Cristina Marques, Sérgio Miguel Pereira, Ruth Gonçalves, Fernando Nunes, Bruno |
| Description | Author Affiliation: Antunes SC ( CESAM, Centro de Estudos Ambientais e do Mar, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro.) |
| Abstract | Antioxidant defences are the primary mechanisms by which organisms compensate oxidative damage caused by contact with several types of substances prone to establish oxidative cycles. Biomarkers are one of the most useful tools to assess effects consequent to environmental exposure to a large number of xenobiotics. However, the use of biomarkers requires previous standardization of enzymatic protocols in order to guarantee accuracy and relevance of obtained results. This article describes standardized conditions for the use of several enzymatic biomarkers (oxidative stress: glutathione peroxidase, lipid peroxidation, glutathione reductase, glutathione-S-transferase and catalase; respiration: lactate dehydrogenase) for the evaluation of responses of specimens of Eisenia andrei, Pelophylax perezi (formerly Rana perezi) and Apodemus sylvaticus. Furthermore, we also present the normal values of activity for the given markers, for non-exposed organisms, that can serve as comparative indexes for subsequent studies. We conclude that these species are thus a suitable target to serve as sentinels in studies of environmental contamination by chemicals, which can lead to oxidative and physiological modifications. |
| File Format | HTM / HTML |
| ISSN | 14640325 |
| Issue Number | 8 |
| Volume Number | 12 |
| e-ISSN | 14640333 |
| Journal | Journal of Environmental Monitoring |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Publisher Date | 2010-08-05 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Subscribed |
| Subject Keyword | Discipline Environmental Health Biological Markers Metabolism Environmental Monitoring Methods Environmental Pollutants Toxicity Animals Catalase Glutathione Peroxidase Glutathione Reductase Glutathione Transferase L-lactate Dehydrogenase Lipid Peroxidation Drug Effects Murinae Oligochaeta Enzymology Ranidae Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law |
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