NDLI: Development of electrochemical based sandwich enzyme linked immunosensor for Cryptosporidium parvum detection in drinking water.

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Development of electrochemical based sandwich enzyme linked immunosensor for Cryptosporidium parvum detection in drinking water.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Thiruppathiraja, Chinnasamy Saroja, Veerappan Kamatchiammal, Senthilkumar Adaikkappan, Periyakaruppan Alagar, Muthukaruppan
Description	Country affiliation: India Author Affiliation: Thiruppathiraja C ( Nanocomposites Research group, Department of Chemical Engineering, Anna University, Chennai, TN, India.)
Abstract	Cryptosporidium parvum is one of the most important biological contaminants in drinking water and generates significant risks to public health. Due to low infectious dose of C. parvum, remarkably sensitive detection methods are required for water and food industry analysis. This present study describes a simple, sensitive, enzyme amplified sandwich form of an electrochemical immunosensor using dual labeled gold nanoparticles (alkaline phosphatase and anti-oocysts monoclonal antibody) in indium tin oxide (ITO) as an electrode to detect C. parvum. The biosensor was fabricated by immobilizing the anti-oocysts McAb on a gold nanoparticle functionalized ITO electrode, followed by the corresponding capture of analytes and dual labeled gold nanoparticle probe to detect the C. parvum target. The outcome shows the sensitivity of electrochemical immune sensor enhanced by gold nanoparticles with a limit of detection of 3 oocysts/mL in a minimal processing period. Our results demonstrated the sensitivity of the new approach compared to the customary method and the immunosensors showed acceptable precision, reproducibility, stability, and could be readily applied to multi analyte determination for environmental monitoring.
File Format	HTM / HTML
ISSN	14640325
Issue Number	10
Volume Number	13
e-ISSN	14640333
Journal	Journal of Environmental Monitoring
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-10-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Environmental Health Cryptosporidium Parvum Growth & Development Drinking Water Microbiology Environmental Monitoring Methods Enzyme-linked Immunosorbent Assay Water Pollution Analysis Isolation & Purification Oocysts Statistics & Numerical Data Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Public Health, Environmental and Occupational Health Management, Monitoring, Policy and Law

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Detection of Cryptosporidium parvum oocysts in bovine feces by monoclonal antibody capture enzyme-linked immunosorbent assay.

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A Nested-PCR Assay for Detection of Cryptosporidium parvum Oocysts in Water Samples

Effect of tillage and rainfall on transport of manure-applied Cryptosporidium parvum oocysts through soil.

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Determination of atrazine in surface waters by combination of POCIS passive sampling and ELISA detection.

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Development of electrochemical based sandwich enzyme linked immunosensor for Cryptosporidium parvum detection in drinking water.

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Development of electrochemical based sandwich enzyme linked immunosensor for Cryptosporidium parvum detection in drinking water.