NDLI: Molecular characterization and environmental mapping of Campylobacter isolates in a subset of intensive poultry flocks in Ireland.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Patriarchi, Alessandro Fox, Aine Maunsell, Bláithín Fanning, Séamus Bolton, Declan
Spatial Coverage	Ireland
Description	Country affiliation: Ireland Author Affiliation: Patriarchi A ( Food Safety Department, Teagasc-Ashtown Food Research Centre, Dublin, Ireland.)
Abstract	Irish breeder and intensive broiler flocks together with the corresponding poultry farm environment were sampled for the presence of Campylobacter with the aim of identifying potential sources and transmission routes of poultry flock contamination. The genetic diversity of a subset of Campylobacter isolates was examined by analysis of the flaA-short variable region (SVR). Additional discrimination for a further subset of these isolates was achieved using multilocus sequence typing (MLST) analysis. Twenty-four flaA-SVR alleles and 15 FlaA peptides were detected among 92 Campylobacter jejuni and Campylobacter coli isolates. MLST data have been determined for 30 of 92 (32.6%) flaA-SVR-typed isolates with 13 sequence types (STs) present, which were assigned to seven clonal complexes. ST45 was the most common ST identified. Vertical transmission was not found to have played a role in transmission of the pathogen to the poultry flocks. Subtyping by flaA-SVR and MLST identified the practice of partial thinning of flocks as a potential source and route of flock contamination on one broiler farm and implicated a probable source of flock contamination on another. Although there have been several studies reported in the scientific literature, the findings from this study confirmed previous studies and suggested some new transmission pathways including via transport crates. Cross-contamination from adjacent cattle is a new development, and molecular evidence of the role of transport crates in introducing Campylobacter spp. into the broiler house is a recent finding. Further, this study reports the discovery of five new flaA-SVR allele types and eight new STs. These were widespread and persistent in the poultry environment. This new knowledge may explain why despite the on-farm Campylobacter data published to date, there are still no completely effective on-farm control measures to prevent Campylobacter contamination of broiler flocks.
File Format	HTM / HTML
ISSN	15353141
Issue Number	1
Volume Number	8
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2011-01-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Campylobacter Infections Veterinary Campylobacter Genetics Chickens Microbiology Poultry Diseases Transmission Animals Bacterial Typing Techniques Biodiversity Classification Isolation & Purification Epidemiology Flagellin Ireland Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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