NDLI: Gilthead seabream (Sparus aurata) as carriers of SHV-12 and TEM-52 extended-spectrum beta-lactamases-containing Escherichia coli isolates.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sousa, Margarida Torres, Carmen Barros, Joana Somalo, Sergio Igrejas, Gilberto Poeta, Patricia
Spatial Coverage	Atlantic Ocean
Description	Country affiliation: Portugal Author Affiliation: Sousa M ( Centre of Studies of Animal and Veterinary Sciences, Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal.)
Abstract	In recent years, bacterial resistance to beta-lactam antibiotics has risen dramatically in Escherichia coli isolated from animals that could pass through the food chain to humans. One hundred eighteen fecal samples of Sparus aurata were tested for extended-spectrum beta-lactamase (ESBL)-containing E. coli recovery. Susceptibility to 16 antimicrobial agents was performed by disk diffusion. ESBL-phenotypic detection was carried out by double-disk test, and the presence of genes encoding TEM, OXA, SHV, and CTX-M type beta-lactamases was studied by polymerase chain reaction and sequencing. The detection of other antimicrobial resistance mechanisms and phylogenetic groups was also performed in recovered isolates as well as their clonal diversity by pulsed-field gel electrophoresis. Five of the 118 fecal samples harbored ESBL-positive E. coli isolates (4.2%), and one isolate per sample was completely characterized. These five ESBL-positive E. coli isolates contained the bla(TEM-52) or bla(SHV-12) genes, as well as a variety of other resistance genes (cmlA, tetA, aadA, sul1, sul2, and sul3). Four isolates harbored class 1 integrons with the following gene cassettes in their variable region: dfrA1 + aadA1 (one isolate) and sat + psp + aadA2 (three isolates). Four unrelated pulsed-field gel electrophoresis patterns were identified among the five ESBL-positive isolates, and they were ascribed to phylogroups A and B1. The intestinal tract of S. aurata might constitute a reservoir of ESBL-producing E. coli isolates.
File Format	HTM / HTML
ISSN	15353141
Issue Number	10
Volume Number	8
e-ISSN	15567125
Journal	Foodborne Pathogens and Disease
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2011-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Microbiology Discipline Parasitology Discipline Nutritional Sciences Escherichia Coli Infections Veterinary Escherichia Coli Enzymology Fish Diseases Microbiology Sea Bream Beta-lactamases Genetics Animals Anti-infective Agents Pharmacology Atlantic Ocean Drug Resistance, Bacterial Electrophoresis, Gel, Pulsed-field Drug Effects Isolation & Purification Escherichia Coli Proteins Metabolism Feces Humans Microbial Sensitivity Tests Phylogeny Polymerase Chain Reaction Beta-lactam Resistance Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Microbiology Applied Microbiology and Biotechnology Food Science

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