NDLI: Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells.

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Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wurm, Melanie Gross, Benjamin Sgodda, Malte Ständker, Ludger Müller, Thomas Forssmann, Wolf-Georg Horn, Peter A. Blasczyk, Rainer Cantz, Tobias
Description	Country affiliation: Germany Author Affiliation: Wurm M ( Institute for Transfusion Medicine, Hannover Medical School, D-30625 Hannover, Germany.)
Abstract	Genetic modification of human embryonic stem cells (hESCs) using biophysical DNA transfection methods are hampered by the very low single cell survival rate and cloning efficiency of hESCs. Lentiviral gene transfer strategies are widely used to genetically modify hESCs but limited transduction efficiencies in the presence of feeder or stroma cells present problems, particularly if vesicular stomatitis virus glycoprotein (VSV-G) pseudotyped viral particles are applied. Here, we investigated whether the recently described semen derived enhancer of virus infection (SEVI) and alternative viral envelope proteins derived from either Gibbon ape leukaemia virus (GALV) or feline leukaemia virus (RD114) are applicable for transducing hESCs during co-culture with feeder or stroma cells. Our first set of experiments demonstrates that SEVI has no toxic effect on murine or hESCs and that exposure to SEVI does not interfere with the pluripotency-associated phenotype. Focusing on hESCs, we were able to further demonstrate that SEVI increases the transduction efficiencies of GALV and RD114 pseudotyped lentiviral vectors. More importantly, aiming at targeted differentiation of hESCs into functional somatic cell types, GALV pseudotyped lentiviral particles could efficiently and exclusively transduce hESCs grown in co-culture with OP9-GFP stroma cells (which were often used to induce differentiation into haematopoietic derivatives).
File Format	HTM / HTML
ISSN	14316730
Issue Number	10
Volume Number	392
e-ISSN	14374315
Journal	Biological Chemistry
Language	English
Publisher	Walter de Gruyter
Publisher Date	2011-10-01
Publisher Place	Germany
Access Restriction	Subscribed
Subject Keyword	Discipline Biochemistry Coculture Techniques Embryonic Stem Cells Metabolism Lentivirus Genetics Leukemia Virus, Gibbon Ape Stromal Cells Transduction, Genetic Methods Viral Envelope Proteins Animals Cell Line Cytology Genetic Vectors Humans Mice Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Biochemistry Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Sequences in the cytoplasmic tail of the gibbon ape leukemia virus envelope protein that prevent its incorporation into lentivirus vectors.

Improved transfer of the leukocyte integrin CD18 subunit into hematopoietic cell lines by using retroviral vectors having a gibbon ape leukemia virus envelope.

Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus Vectors

RD114 envelope proteins provide an effective and versatile approach to pseudotype lentiviral vectors.

Improved transfer of the leukocyte integrin CD18 subunit into hematopoietic cell lines by using retroviral vectors having a gibbon ape leukemia virus envelope

The derivation of mesenchymal stem cells from human embryonic stem cells.

Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteins.

Stable transduction of quiescent CD34(+)CD38(-) human hematopoietic cells by HIV-1-based lentiviral vectors.

Viral and cellular factors governing hamster cell infection by murine and gibbon ape leukemia viruses (1991)

Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells.

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Sequences in the cytoplasmic tail of the gibbon ape leukemia virus envelope protein that prevent its incorporation into lentivirus vectors.

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Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus Vectors

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Improved lentiviral gene transfer into human embryonic stem cells grown in co-culture with murine feeder and stroma cells.