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Year: 2010 Volume: 12 Issue: 2

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Molecular heterogeneity of gelatin-binding proteins from human seminal plasma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kosanovic, Maja M. Jankovic, Miroslava M.
Description	Author Affiliation: Kosanovic MM ( Department for Immunochemistry and Glycobiology, Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Banatska 31b, Zemun-Belgrade 11080, Serbia. miraj@inep.co.rs)
Abstract	Defining the molecular characteristics of seminal plasma proteins is essential for understanding their function in physiological and pathological conditions. Starting from the predicted importance of human seminal plasma gelatin-binding proteins, comprising fibronectin (FN) and FN-related molecules, for male fertility, this study aims at gaining insight into their immuno-glycobiochemical properties. Human seminal plasma from subjects with normal semen parameters were separated on a gelatin-Sepharose column and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting using antibodies against distinct FN forms. Heterogeneity of the isolated molecular species was examined by protein chip arrays combined with surface-enhanced laser desorption/ionization time of flight mass spectrometry, on normal, metal and hydrophobic surfaces. Carbohydrate composition was investigated using mannose-, fucose- and sialic acid-specific plant lectins and galectin-1. The results obtained indicated a pattern of isolated proteins corresponding to that of known FN fragments, as confirmed by immunoreactivity. Among them heparin-binding ability was preferentially associated with low molecular mass species. As for posttranslational modifications, phosphorylation and glycosylation of distinct fragments were revealed. Lectin binding to fragments containing the gelatin-binding domain, particularly with Ricinus communis agglutinin I, was stronger than to fragments containing the cell-binding site of FN. A low level of sialylation and distinctive concanavalin A- and Lens culinaris agglutinin-reactive species were also observed. Galectin-1 did not interact with the isolated preparation. Resolving the molecular heterogeneity of normal human seminal plasma FN and gaining initial insight into possible similarities/differences with known FN molecular species may be considered a prerequisite step preceding challenging the clinical usefulness of these molecular properties.
File Format	HTM / HTML
ISSN	1008682X
e-ISSN	17457262
DOI	10.1038/aja.2009.98
Journal	Asian Journal of Andrology
Issue Number	3
Volume Number	12
Language	English
Publisher	Wolters Kluwer - Medknow
Publisher Date	2010-05-01
Publisher Place	China
Access Restriction	Open
Subject Keyword	Discipline Urology Fibronectins Metabolism Semen Seminal Plasma Proteins Chromatography, Agarose Electrophoresis, Polyacrylamide Gel Galectin 1 Chemistry Glycosylation Heparin Phosphorylation Plant Lectins Protein Array Analysis Protein Binding Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Urology

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Molecular heterogeneity of gelatin-binding proteins from human seminal plasma

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