| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Liu, Xin-yu Zhou, Xin-yu Hou, Jin-cai Zhu, Hua Wang, Zhong Liu, Jian-xun Zheng, Yong-qiu |
| Description |
Country affiliation: China Author Affiliation: Liu XY ( 1] Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China [2] Beijing University of Chinese Medicine, Beijing 100029, China.); Zhou XY ( Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.); Hou JC ( Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.); Zhu H ( Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.); Wang Z ( Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100029, China.); Liu JX ( Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China.); Zheng YQ ( 1] Institute of Basic Medical Sciences of Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China [2] Department of Surgery, Davis Heart and Lung Research Institute, Ohio State University Wexner Medical Center, Columbus, OH 43210, USA.) |
| Abstract |
Aim: To investigate the effects of ginsenoside Rd (Rd) on neurogenesis in rat brain after ischemia/reperfusion injury (IRI). Methods: Male SD rats were subjected to transient middle cerebral artery occlusion (MCAO) followed by reperfusion. The rats were injected with Rd (1, 2.5, and 5 $mg·kg^{−1}·d^{−1},$ ip) from d 1 to d 3 after MCAO, and with BrdU (50 $mg·kg^{−1}·d^{−1},$ ip) from d 3 to d 6, then sacrificed on 7 d. The infarct size and neurological scores were assessed. Neurogenesis in the brains was detected by BrdU, DCX, Nestin, and GFAP immunohistochemistry staining. PC12 cells subjected to OGD/reperfusion were used as an in vitro model of brain ischemia. VEGF and BDNF levels were assessed with ELISA, and Akt and ERK phosphorylation was measured using Western blotting. Results: Rd administration dose-dependently decreased the infarct size and neurological scores in the rats with IRI. The high dose of Rd 5 $(mg·kg^{−1}·d^{−1})$ significantly increased Akt phosphorylation in ipsilateral hemisphere, and markedly increased the number of BrdU/DCX and Nestin/GFAP double-positive cells in ischemic area, which was partially blocked by co-administration of the PI3 kinase inhibitor LY294002. Treatment with Rd (25, 50, and 100 μmol/L) during reperfusion significantly increased the expression of VEGF and BDNF in PC12 cells with IRI. Furthermore, treatment with Rd dose-dependently increased the phosphorylation of Akt and ERK, and significantly decreased PC12 cell apoptosis, which were blocked by co-application of LY294002. Conclusion: Rd not only attenuates ischemia/reperfusion injury in rat brain, but also promotes neurogenesis via increasing VEGF and BDNF expression and activating the PI3K/Akt and ERK1/2 pathways. |
| File Format |
HTM / HTML |
| ISSN |
16714083 |
| e-ISSN |
17457254 |
| DOI |
10.1038/aps.2014.156 |
| Journal |
Acta Pharmacologica Sinica |
| Issue Number |
4 |
| Volume Number |
36 |
| Language |
English |
| Publisher |
Macmillan Publishers Limited |
| Publisher Date |
2015-04-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Pharmacology Brain Blood Supply Ginsenosides Therapeutic Use Infarction, Middle Cerebral Artery Drug Therapy Ischemic Attack, Transient Neurogenesis Drug Effects Phosphatidylinositol 3-kinases Metabolism Proto-oncogene Proteins C-akt Animals Pathology Brain-derived Neurotrophic Factor Chemistry Map Kinase Signaling System Panax Rats, Sprague-dawley Reperfusion Injury Signal Transduction Vascular Endothelial Growth Factor A Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Pharmacology Pharmacology (medical) |
