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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nakamura, Hiroyuki Wakita, Shigeo Suganami, Akiko Tamura, Yutaka Hanada, Kentaro Murayama, Toshihiko |
| Description | Country affiliation: Japan Author Affiliation: Nakamura H ( Laboratory of Chemical Pharmacology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan. ropi@p.chiba-u.ac.jp) |
| Abstract | We examined the effect of the cellular sphingolipid level on the release of arachidonic acid (AA) and activity of cytosolic phospholipase A2alpha (cPLA2alpha) using two Chinese hamster ovary (CHO)-K1-derived mutants deficient in sphingolipid synthesis: LY-B cells defective in the LCB1 subunit of serine palmitoyltransferase for de novo synthesis of sphingolipid species, and LY-A cells defective in the ceramide transfer protein CERT for SM synthesis. When LY-B and LY-A cells were cultured in Nutridoma medium and the sphingolipid level was reduced, the release of AA stimulated by the Ca(2+) ionophore A23187 increased 2-fold and 1.7-fold, respectively, compared with that from control cells. The enhancement in LY-B cells was decreased by adding sphingosine and treatment with the cPLA2alpha inhibitor. When CHO cells were treated with an acid sphingomyelinase inhibitor to increase the cellular SM level, the release of AA induced by A23187 or PAF was decreased. In vitro studies were then conducted to test whether SM interacts directly with cPLA2alpha. Phosphatidylcholine vesicles containing SM reduced cPLA2alpha activity. Furthermore, SM disturbed the binding of cPLA2alpha to glycerophospholipids. These results suggest that SM at the biomembrane plays important roles in regulating the cPLA2alpha-dependent release of AA by inhibiting the binding of cPLA2alpha to glycerophospholipids. |
| File Format | HTM / HTML |
| ISSN | 00222275 |
| e-ISSN | 15397262 |
| DOI | 10.1194/jlr.M002428 |
| Journal | The Journal of Lipid Research |
| Issue Number | 4 |
| Volume Number | 51 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2010-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Biochemistry Arachidonic Acid Metabolism Group Iv Phospholipases A2 Antagonists & Inhibitors Sphingolipids Sphingomyelins Animals Cho Cells Cell Line Cricetinae Cricetulus Enzyme Inhibitors Pharmacology Extracellular Signal-regulated Map Kinases Glycerophospholipids Green Fluorescent Proteins Genetics Liposomes Mutation Phosphorylation Drug Effects Protein Binding Protein Transport Recombinant Fusion Proteins Biosynthesis Sphingosine Analogs & Derivatives Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Endocrinology |
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