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High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jiang, Haibo Goulbourne, Chris N. Tatar, Angelica Turlo, Kirsten Wu, Daniel Beigneux, Anne P. Grovenor, Chris R. M. Fong, Loren G. Young, Stephen G.
Description	Country affiliation: United kingdom Author Affiliation: Jiang H ( Materials Department, Oxford University, Oxford, United Kingdom.); Goulbourne CN ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Tatar A ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Turlo K ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Wu D ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Beigneux AP ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Grovenor CR ( Materials Department, Oxford University, Oxford, United Kingdom.); Fong LG ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA.); Young SG ( Departments of Medicine University of California, Los Angeles, Los Angeles, CA Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA.)
Abstract	Nanoscale secondary ion MS (NanoSIMS) imaging makes it possible to visualize stable isotope-labeled lipids in cells and tissues at 50 nm lateral resolution. Here we report the use of NanoSIMS imaging to visualize lipids in mouse cells and tissues. After administering stable isotope-labeled fatty acids to mice by gavage, NanoSIMS imaging allowed us to visualize neutral lipids in cytosolic lipid droplets in intestinal enterocytes, chylomicrons at the basolateral surface of enterocytes, and lipid droplets in cardiomyocytes and adipocytes. After an injection of stable isotope-enriched triglyceride-rich lipoproteins (TRLs), NanoSIMS imaging documented delivery of lipids to cytosolic lipid droplets in parenchymal cells. Using a combination of backscattered electron (BSE) and NanoSIMS imaging, it was possible to correlate the chemical data provided by NanoSIMS with high-resolution BSE images of cell morphology. This combined imaging approach allowed us to visualize stable isotope-enriched TRLs along the luminal face of heart capillaries and the lipids within heart capillary endothelial cells. We also observed examples of TRLs within the subendothelial spaces of heart capillaries. NanoSIMS imaging provided evidence of defective transport of lipids from the plasma LPs to adipocytes and cardiomyocytes in mice deficient in glycosylphosphatidylinositol-anchored HDL binding protein 1.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M053363
Journal	The Journal of Lipid Research
Issue Number	10
Volume Number	55
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2014-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Adipocytes Metabolism Cytosol Dietary Fats Endothelial Cells Molecular Imaging Myocytes, Cardiac Cytology Animals Lipoproteins Mice Mice, Knockout Triglycerides Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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High-resolution imaging of dietary lipids in cells and tissues by NanoSIMS analysis.