NDLI: Separation and quantification of 2-acyl-1-lysophospholipids and 1-acyl-2-lysophospholipids in biological samples by LC-MS/MS.

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Separation and quantification of 2-acyl-1-lysophospholipids and 1-acyl-2-lysophospholipids in biological samples by LC-MS/MS.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Okudaira, Michiyo Inoue, Asuka Shuto, Akira Nakanaga, Keita Kano, Kuniyuki Makide, Kumiko Saigusa, Daisuke Tomioka, Yoshihisa Aoki, Junken
Description	Country affiliation: Japan Author Affiliation: Okudaira M ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.); Inoue A ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan PRESTOJapan Science and Technology Corporation (JST), Kawaguchi, Saitama, 332-0012, Japan.); Shuto A ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.); Nakanaga K ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.); Kano K ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.); Makide K ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan PRESTOJapan Science and Technology Corporation (JST), Kawaguchi, Saitama, 332-0012, Japan.); Saigusa D ( CREST, Japan Science and Technology Corporation (JST), Kawaguchi, Saitama, 332-0012, Japan Department of Integrative Genomics, Tohoku Medical Megabank, Tohoku University, 2-1, Seiryou-machi, Aoba-ku, Sendai, Miyagi, 980-8573, Japan.); Tomioka Y ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan.); Aoki J ( Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Aoba-ku, Sendai, Miyagi, 980-8578, Japan CREST, Japan Science and Technology Corporation (JST), Kawaguchi, Saitama, 332-0012, Japan.)
Abstract	Lysophospholipids (LysoGPs) serve as lipid mediators and precursors for synthesis of diacyl phospholipids (GPs). LysoGPs detected in cells have various acyl chains attached at either the sn-1 or sn-2 position of the glycerol backbone. In general, acyl chains at the sn-2 position of 2-acyl-1-LysoGPs readily move to the sn-1 position, generating 1-acyl-2-lyso isomers by a nonenzymatic reaction called intra-molecular acyl migration, which has hampered the detection of 2-acyl-1-LysoGPs in biological samples. In this study, we developed a simple and versatile method to separate and quantify 2-acyl-1- and 1-acyl-2-LysoGPs. The main point of the method was to extract LysoGPs at pH 4 and 4°C, conditions that were found to completely eliminate the intra-molecular acyl migration. Under the present conditions, the relative amounts of 2-acyl-1-LysoGPs and 1-acyl-2-LysoGPs did not change at least for 1 week. Further, in LysoGPs extracted from cells and tissues under the present conditions, most of the saturated fatty acids (16:0 and 18:0) were found in the sn-1 position of LysoGPs, while most of the PUFAs (18:2, 20:4, 22:6) were found in the sn-2 position. Thus the method can be used to elucidate the in vivo role of 2-acyl-1-LysoGPs.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.D048439
Journal	The Journal of Lipid Research
Issue Number	10
Volume Number	55
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2014-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Lysophospholipids Chemistry Isolation & Purification Animals Chromatography, Liquid Hek293 Cells Mass Spectrometry Mice Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Endocrinology

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